Keratin 17 and Collagen type 1 genes: Esophageal cancer molecular marker discovery and evaluation

• Published in: The clinical respiratory journal Vol. 18; no. 7; pp. e13793 - n/a
• Main Authors: Pan, Huiwen, Hong, Jie, Shao, Aizhong, Zhao, Zhiguo, Ding, Guowen, Fang, Zhijie, Chen, Keping, Zhu, Jingfeng
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.07.2024; John Wiley and Sons Inc; Wiley
• Subjects: Aged; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; COL1A1; Collagen Type I - genetics; Collagen Type I - metabolism; Collagen Type I, alpha 1 Chain; combined detection; Esophageal cancer; Esophageal Neoplasms - genetics; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma - genetics; Esophageal Squamous Cell Carcinoma - metabolism; Female; Gene Expression Regulation, Neoplastic; Humans; Keratin-17 - genetics; Keratin-17 - metabolism; KRT17; Male; marker; Middle Aged; Original; COL1A1; combined detection; esophageal cancer; KRT17; marker
• ISSN: 1752-6981; 1752-699X; 1752-699X
• DOI: 10.1111/crj.13793

ABSTRACT One hundred eighty pairs of tissues of esophageal squamous cell carcinoma (ESCC) were tested by the transcriptome sequencing in order to explore etiology factors. The chi‐square test and correlation analysis demonstrated that the relative expression levels of keratin 17 (KRT17) and collagen type I α1 chain (COL1A1) were significantly higher in EC with diabetes. Expression of KRT17 was correlated with blood glucose (r = 0.204, p = 0.001) and tumor size (r = −0.177, p = 0.038) in patients. COL1A1 correlated with age (r = −0.170, p = 0.029) and blood glucose levels (r = 0.190, p = 0.015). Experimental results of qRT‐PCR: KRT17 and COL1A1 genes were highly expressed in ESCC (p < 0.05). When the two genes were used as a combination test, the positive detection rate of EC was 90.6%, and the ROC curve had greater power. The KRT17 and COL1A1 genes had the potential to be biomarkers for the diagnosis of ESCC. Testing the KRT17 gene and the COL1A1 gene from 180 paired ESCC tissues by using transcriptome technology, para‐cacinoma tissues as a control group and carcinoma tissues as a case group. Recording every factor, including patient sex, age, smoking, alcohol, blood glucose, and tumor size, from the electronic medical system. Analyzing experimental data from two groups, both KRT17 and COL1A1 mRNA were highly expressed in case tissues, which are also correlated with age, blood glucose and tumor size. KRT17 and COL1A1 are potential biomarkers for esophageal cancer in the Chinese population.