Identification of sea urchin gonads chemical compounds using thin-layer chromatography from Bokory island, Southeast Sulawesi

Abstract Southeast Sulawesi is one area that has a diversity of potential marine biota as medicinal ingredients, including the sea urchin gonads. Empirically coastal communities have been hereditary, consuming sea urchin gonads as a source of high protein. Considering its potential as a raw material...

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Bibliographic Details
Published inJournal of physics. Conference series Vol. 1899; no. 1; pp. 12050 - 12058
Main Authors Karmilah, Musdalipah, Daud, Nur Saadah, Reymon, Fauziah, Yulianti
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.05.2021
Subjects
Alkaloids
Chemical compounds
Chromatography
Design of experiments
Drying
Ethanol
Ethyl acetate
Gonads
Invertebrates
Metabolites
Physics
Phytochemicals
Saponins
Screening
Thin layer chromatography
Sulawesi
Indonesia
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Summary:Abstract Southeast Sulawesi is one area that has a diversity of potential marine biota as medicinal ingredients, including the sea urchin gonads. Empirically coastal communities have been hereditary, consuming sea urchin gonads as a source of high protein. Considering its potential as a raw material for medicines, it is necessary to conduct a series of tests to guarantee its safety and efficacy. This study aims to determine the chemical compounds of sea urchin gonad extract ( Diadema setosum L) by Thin-Layer Chromatography (TLC). The research method uses a Pre-experimental design with a one-shot case study model. Identification of metabolites of sea urchin gonad extracts was carried out in two stages: phytochemical screening and identification by TLC method by UV-Vis. Samples were dried by the Freeze-drying method to get 335.11 g of dry samples and 58.06 g of extracts. The results of the test of the content of water-soluble and ethanol-soluble sea urchin extract were 5.73% and 3.72%. The results showed that in phytochemical screening, ethyl acetate extracts of the positive marine gonads contained alkaloids, saponins, and steroids. In addition, UV-vis 254 nm and 366 nm TLC methods showed alkaloids and saponins. The Rf values are 0.4 and 0.68, respectively.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/1899/1/012050