Antioxidant, Antimicrobial, Antidiabetic and Cytotoxic Activity of Crocus sativus L. Petals

• Published in: Applied sciences Vol. 10; no. 4; p. 1519
• Main Authors: Wali, Adil Farooq, Alchamat, Houda Ahmed Abou, Hariri, Huda Khaled, Hariri, Bushra Khaled, Menezes, Godfred A., Zehra, Uzma, Rehman, Muneeb U., Ahmad, Parvaiz
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.02.2020
• Subjects: abts assay; anti-diabetic; Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Antioxidants; Cell lines; Crocus sativus; crocus sativus l. petals; cytotoxic; Cytotoxicity; Diabetes mellitus; Ethanol; Evaluation; Flavonoids; ftir; Glucosidase; Gram-positive bacteria; Herbal medicine; Infectious diseases; Minimum inhibitory concentration; Petals; polyphenols; Saffron; Scavenging; Sulfonic acid; α-Glucosidase; United States > US
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app10041519

The purpose of this research is to examine in vitro antioxidant, antimicrobial, antidiabetic and cytotoxic efficacy of different extracts of Crocus sativus L. petals. Antioxidant activity of extracts was assessed by DPPH and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assay. Among all extracts, ethanol (SPE) had the maximum radical scavenging activity with IC50 values 86.63 ± 7.53 μg/mL. The antimicrobial activity was determined by the evaluation of the minimum inhibitory concentration using the agar well plate procedure. The most effective extract was SPE with a minimum inhibitory concentration varying between 500 µg/mL, 250 µg/mL, 125 µg/mL, 62.5 µg/mL, 31.25 µg/mL, 15.63 µg/mL. Cytotoxic activity was tested against MDA-MB-231 cell lines using the MTT method whereas, antidiabetic activity was evaluated using an alpha-glucosidase inhibition assay. All extracts were found to have significant antidiabetic activity.