Capillary-driven microfluidic device with integrated nanoporous microbeads for ultrarapid biosensing assays

[Display omitted] •A single-step capillary-driven microfluidic biosensing device was developed.•Commercial protein A beads served as support to perform a competitive immunoassay.•The strictly regulated mycotoxin deoxynivalenol was tested as a model analyte.•The device included an internal reference...

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Bibliographic Details
Published inSensors and actuators. B, Chemical Vol. 265; pp. 452 - 458
Main Authors Epifania, Roberta, Soares, Ruben R.G., Pinto, Inês F., Chu, Virginia, Conde, João P.
Format Journal Article
LanguageEnglish
Published Lausanne Elsevier B.V 15.07.2018
Elsevier Science Ltd
Subjects
Beads
Biosensors
Capillarity
Fluorescence
Immunoassay
Microfluidics
Mycotoxins
Nanoparticles
Product safety
Rapid
Toxins
Immunoassay
Beads
Capillarity
Rapid
Microfluidics
Mycotoxins
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Summary:[Display omitted] •A single-step capillary-driven microfluidic biosensing device was developed.•Commercial protein A beads served as support to perform a competitive immunoassay.•The strictly regulated mycotoxin deoxynivalenol was tested as a model analyte.•The device included an internal reference and used only 4.5 μL of sample.•Minimum concentrations of 1.7 ng/mL deoxynivalenol were detected in 70 s assay time. For microfluidic devices to achieve a practical point-of-need application, it is necessary to circumvent the often excessive system complexity required, such as external pumps and multi-step operation, in order to provide a rapid and simple, while still robust and fit-for-purpose device. We report a simple capillary-based microfluidic device with integrated microbeads and a no-wash, single-step mode of operation that achieves a sub-minute detection of analytes using a fluorescent competitive immunoassay. In particular, minimum detectable limits of 1.7 ng/mL were obtained for mycotoxin detection within 70 s assay time, using 4.5 μL of sample. Furthermore, an internal control was also included in the microfluidic device to provide additional robustness and result validation. These results support the development of ultra-rapid and simple microfluidic devices, easily extended to other relevant targets within the food safety, biomedical or environmental fields.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2018.03.051