Exogenous ethylene reduces growth via alterations in central metabolism and cell wall composition in tomato (Solanum lycopersicum)

• Published in: Journal of plant physiology Vol. 263; p. 153460
• Main Authors: Nascimento, Vitor L., Pereira, Auderlan M., Siqueira, João Antônio, Pereira, Aurelio S., Silva, Victor F., Costa, Lucas C., Ribeiro, Dimas M., Zsögön, Agustin, Nunes-Nesi, Adriano, Araújo, Wagner L.
• Format: Journal Article
• Language: English
• Published: Stuttgart Elsevier GmbH 01.08.2021; Elsevier Science Ltd
• Subjects: Assimilation; Biosynthesis; Carbohydrates; Carbon; Carbon metabolism; Cell walls; Cellulose; Chloroethylphosphonic acid; Ethephon; Ethylene; Gas exchange; Growth inhibition; Leaf area; Leaves; Metabolic response; Metabolism; Phenols; Phenotypes; Photosynthesis; Plant growth; Solanum lycopersicum; Stomata; Stomatal conductance; Tomato leaves; Tomatoes; Carbon metabolism; Gas exchange; Ethephon; Growth inhibition; Tomato leaves
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2021.153460

Ethylene is a gaseous hormone with a well-established role in the regulation of plant growth and development. However, its role in the modulation of carbon assimilation and central metabolism remains unclear. Here, we investigated the morphophysiological and biochemical responses of tomato plants (Solanum lycopersicum) following the application of ethylene in the form of ethephon (CEPA - 2-chloroethylphosphonic acid), forcing the classical triple response phenotype. CEPA-treated plants were characterized by growth inhibition, as revealed by significant reductions in both shoot and root dry weights, coupled with a reduced number of leaves and lower specific leaf area. Growth inhibition was associated with a reduction in carbon assimilation due to both lower photosynthesis rates and stomatal conductance, coupled with impairments in carbohydrate turnover. Furthermore, exogenous ethylene led to the accumulation of cell wall compounds (i.e., cellulose and lignin) and phenolics, indicating that exposure to exogenous ethylene also led to changes in specialized metabolism. Collectively, our findings demonstrate that exogenous ethylene disrupts plant growth and leaf structure by affecting both central and specialized metabolism, especially that involved in carbohydrate turnover and cell wall biosynthesis, ultimately leading to metabolic responses that mimic stress situations. •Exogenous ethylene reduces growth and affects growth-related parameters in tomato.•C assimilation (and growth) decreases because lower gas exchange and impairments in carbohydrate turnover.•Ethylene treatment alters cell wall composition (cellulose and lignin) and total phenolics levels.•Exogenous ethylene disrupts plant growth and ultimately lead to metabolic responses that mimic stress situations.•Ethylene may play a more direct role in plant metabolism most likely through effects on C assimilation and metabolism.