A label-free colorimetric assay for detection of c-Myc mRNA based on peptide nucleic acid and silver nanoparticles

A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles (PNA/AgNPs) has been initially proposed for specific recognition of mRNA. Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc mRNA detection can be...

Full description

Saved in:
Bibliographic Details
Published inScience bulletin (Beijing) Vol. 61; no. 4; pp. 276 - 281
Main Authors Li, Xia, Song, Juan, Chen, Bao-Li, Wang, Bing, Li, Rui, Jiang, Hui-Min, Liu, Ji-Feng, Li, Chen-Zhong
Format Journal Article
LanguageEnglish
Published Beijing Elsevier B.V 01.02.2016
Science China Press
Subjects
c-Myc mRNA
Chemistry/Food Science
color
Colorimetric
colorimetry
Earth Sciences
Engineering
Humanities and Social Sciences
Life Sciences
messenger RNA
multidisciplinary
nanosilver
Peptide nucleic acid
Physics
proto-oncogenes
Science
Science (multidisciplinary)
Silver nanoparticles
Peptide nucleic acid
c-Myc mRNA
Silver nanoparticles
Colorimetric
比色法
mRNA
肽核酸
纳米银
Online AccessGet full text

Cover

More Information
Summary:A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles (PNA/AgNPs) has been initially proposed for specific recognition of mRNA. Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc mRNA detection can be achieved. Moreover, the PNA/AgNPs platform can undergo color change in response to target c-Myc mRNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.
Bibliography:http://dx.doi.org/10.1007/s11434-016-1004-3
ISSN:2095-9273
2095-9281
DOI:10.1007/s11434-016-1004-3