Nonomuraea wenchangensis sp. nov., isolated from mangrove rhizosphere soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 61; no. Pt 6; pp. 1304 - 1308
• Main Authors: FAN WANG, XU, Xiao-Xiong, ZHI QU, CHENG WANG, LIN, Hai-Peng, XIE, Qing-Yi, RUAN, Ji-Sheng, MING SUN, KUI HONG
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.06.2011
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - physiology; Aerobiosis; Bacteriology; Biological and medical sciences; Carbohydrates - analysis; Cell Wall - chemistry; Cluster Analysis; Diaminopimelic Acid - analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Muramic Acids - analysis; Nucleic Acid Hybridization; Phospholipids - analysis; Phylogeny; Rhizophoraceae - growth & development; Rhizosphere; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Systematics; Vitamin K 2 - analysis; Rhizosphere; Soils; Bacteria; New species; Mangrove; Taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.025742-0

Strain 210417(T), which forms highly branched substrate and aerial mycelia, is a Gram-positive, aerobic and non-motile actinomycete isolated from mangrove rhizosphere soil. 16S rRNA gene sequence analysis showed that the strain should be classified in the genus Nonomuraea, being most closely related to the type strains of Nonomuraea coxensis (99.6 %) and Nonomuraea bangladeshensis (99.3 %). Chemotaxonomic properties [madurose as the major sugar in the cell wall; meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan; MK-9(H(4)) as the major menaquinone; iso-C(16 : 0) (24.1 %) as major fatty acid; and phospholipid pattern type IV] are consistent with the assignment of strain 210417(T) to the genus Nonomuraea. Strain 210417(T) could be differentiated from the closely related species N. coxensis and N. bangladeshensis by morphological, physiological, biochemical and chemotaxonomic properties, 16S rRNA gene sequence analysis and DNA-DNA hybridization results. It is therefore proposed that strain 210417(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea wenchangensis sp. nov. is given; the type strain is 210417(T) ( = CGMCC 4.5598(T)  = DSM 45477(T)).