Soil Microbiome Study Based on DNA Extraction: A Review

In recent years, many different methods that allow for the analysis of the biodiversity and structure of the community of microorganisms inhabiting the soil environment have emerged. Many of these approaches are based on molecular methods including the study of genetic biodiversity based on DNA and...

Full description

Saved in:
Bibliographic Details
Published inWater (Basel) Vol. 14; no. 24; p. 3999
Main Author Wydro, Urszula
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.12.2022
Subjects
Acids
Biodiversity
Bioinformatics
DNA
edaphic factors
Efficiency
Genetic diversity
Laboratories
Methods
Microbial genetics
Molecular biology
Polyethylene glycol
Polymorphism
RNA
soil
soil analysis
Soil microbiology
Soil microorganisms
Soil research
water
Poland
Online AccessGet full text

Cover

More Information
Summary:In recent years, many different methods that allow for the analysis of the biodiversity and structure of the community of microorganisms inhabiting the soil environment have emerged. Many of these approaches are based on molecular methods including the study of genetic biodiversity based on DNA and RNA analysis. They are superior to conventional methods because they do not rely on time-consuming laboratory in vitro cultures and biochemical analyses. Moreover, methods based on the analysis of genetic material are characterized by high sensitivity and repeatability. The abovementioned issues are the subject of many reviews. The novelty of this article is the summarization of the main aspects of soil biological research including genetic techniques, bioinformatics and statistical tools. This approach could be an introduction for scientists starting their work in the field of genetic soil analysis. Additionally, examples of the application of molecular methods in soil research are presented.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2073-4441
2073-4441
DOI:10.3390/w14243999