Shinella daejeonensis sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant

• Published in: International journal of systematic and evolutionary microbiology Vol. 61; no. Pt 9; pp. 2123 - 2128
• Main Authors: LEE, Myungjin, WOO, Sung-Geun, TEN, Leonid N
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.09.2011
• Subjects: Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Korea; Locomotion; Microbiology; Molecular Sequence Data; Nitrates - metabolism; Nucleic Acid Hybridization; Oxidation-Reduction; Phylogeny; Quinones - analysis; Rhizobiaceae - classification; Rhizobiaceae - genetics; Rhizobiaceae - isolation & purification; Rhizobiaceae - physiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sewage - microbiology; Systematics; Korea; Bacteria; New species; Nitrates; Treatment; Taxonomy; Leachate
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.026435-0

A Gram-negative-staining, motile, rod-shaped, aerobic bacterial strain, designated MJ02(T), was isolated from sludge of a leachate treatment plant in Daejeon (South Korea) and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ02(T) belonged to the family Rhizobiaceae, class Alphaproteobacteria, and was most closely related to Shinella yambaruensis MS4(T) (97.6 % sequence similarity) and Shinella fusca DC-196(T) (97.5 %). The G+C content of the genomic DNA of strain MJ02(T) was 64.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone and a fatty acid profile with C₁₈:₁ω7c (45.8 %) and C₁₆:₀ (21.8 %) as the major components supported the affiliation of strain MJ02(T) to the genus Shinella. However, strain MJ02(T) exhibited relatively low levels of DNA-DNA relatedness with respect to S. fusca DSM 21319(T) (17±7 %) and S. yambaruensis KACC 14483(T) (12±6 %), showing clearly that the isolate constituted a new genospecies. Strain MJ02(T) could be clearly differentiated from its phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ02(T) is considered to represent a novel species of the genus Shinella, for which the name Shinella daejeonensis sp. nov. is proposed. The type strain is MJ02(T) ( = KCTC 22450(T) = JCM 16236(T)).