Glucocorticoid receptors involved in melatonin inhibiting cell apoptosis and NLRP3 inflammasome activation caused by bacterial toxin pyocyanin in colon

• Published in: Free radical biology & medicine Vol. 162; pp. 478 - 489
• Main Authors: Cai, Liuping, Chen, Qu, Yao, Zhihao, Sun, Qinwei, Wu, Lei, Ni, Yingdong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2021
• Subjects: Animals; Apoptosis; Bacterial Toxins; Colon; Glucocorticoid receptors (GR); Goblet cell; Humans; Inflammasomes; Inflammation; Melatonin (MEL); Melatonin - pharmacology; Mice; Mitochondria; NLR Family, Pyrin Domain-Containing 3 Protein - genetics; NLRP3 inflammasome; Pyocyanin (PCN); Pyocyanine; Reactive Oxygen Species; Receptors, Glucocorticoid - genetics; ROS; Goblet cell; Mitochondria; ROS; Melatonin (MEL); Inflammation; Colon; Glucocorticoid receptors (GR); NLRP3 inflammasome; Pyocyanin (PCN); Apoptosis
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2020.11.003

The immunoinhibitory effect of glucocorticoid and immunoenhancing attributes of melatonin (MEL) are well known, however, the involvement of glucocorticoid receptor (GR) in melatonin modulation of bacterial toxins caused-inflammation has not been studied in colon. Pyocyanin (PCN), a toxin released by Pseudomonas aeruginosa, can destroy cells through generating superoxide products and inflammatory response. Here we report that PCN treatment elevated the generation of reactive oxygen species (ROS), which further lead to mitochondrial swelling and caspase cascades activation both in vivo and in vitro. However, MEL treatment alleviated the oxidative stress caused by PCN on cells through scavenging ROS and restoring the expression of antioxidant enzyme so that to effectively alleviate the apoptosis. Large amounts of ROS can activate the NLRP3 signaling pathway, so MEL inhibited PCN induced NLRP3 inflammasome activation and inflammatory cytokines (IL-1β, IL-8, and TNF-α) secretion. In order to further investigate the molecular mechanism, goblet cells were exposed to MEL and PCN in the presence of luzindole and RU486, inhibitors of MEL receptors and GR respectively. It was found that PCN significantly inhibited the expression level of GR, and MEL effectively alleviated the inhibition phenomenon. Moreover, we found that MEL mainly upregulated the expression of GR to achieve its anti-inflammatory and anti-apoptotic functions rather than through its own receptor (MT2) in colon goblet cells. Therefore, MEL can reverse the inhibitory effects of PCN on GR/p-GR expression to present its anti-oxidative and anti-apoptotic function. [Display omitted] •Glucocorticoid receptors are the vital targets of bacterial toxins pyocyanin in colon.•Melatonin presented anti-apoptotic effects mainly through glucocorticoid receptors.•Glucocorticoid receptors were involved in melatonin inhibiting the activation of NLRP3 inflammasome induced by pyocyanin.