Biomechanical properties of canine corticocancellous bone frozen in normal saline solution

• Published in: American journal of veterinary research Vol. 56; no. 6; p. 822
• Main Authors: Griffon, D.J. (University of Minnesota, St. Paul, MN.), Wallace, L.J, Bechtold, J.E
• Format: Journal Article
• Language: English
• Published: United States 01.06.1995
• Subjects: ALMACENAMIENTO; Animals; Biomechanical Phenomena; Bone and Bones - physiology; Bone Transplantation - veterinary; CHIEN; CHULETA; CONGELACION; CONGELATION; COTELETTE; Cryopreservation; Dogs; HUESOS DE LOS MIEMBROS; Metacarpus - physiology; Organ Preservation; OS DES MEMBRES; PERRO; RESISTANCE MECANIQUE; RESISTENCIA MECANICA; SOLUCION; SOLUTION; STOCKAGE; Stress, Mechanical; Transplantation, Homologous
• ISSN: 0002-9645; 1943-5681
• DOI: 10.2460/ajvr.1995.56.06.822

Eleven pairs of canine metacarpal bones, 10 pairs of metatarsal bones, and 7 pairs of ribs were harvested cleanly and prepared for banking at -20 C for 1 year. One bone of each pair was randomly assigned to 1 type of storage: plastic pack vs immersion in a normal solution of sodium chloride. The contralateral bone was assigned to the opposite treatment. Six pairs of metacarpal bones and 5 pairs of metatarsal bones were tested in torsion to failure. No significant difference was found within pairs. All ribs, 5 pairs of metacarpal bones, and 5 pairs of metatarsal bones were loaded in 4-point bending to failure. The energy absorbed at failure and the ultimate displacement of ribs and metacarpal and metatarsal bones were increased by 25 to 30% and 18 to 24%, respectively, when the bones were frozen in isotonic saline solution. Corticocancellous grafts frozen in normal saline solution are biomechanically less fragile and brittle than grafts stored in plastic without saline solution