Marmoricola korecus sp. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 61; no. Pt 7; pp. 1628 - 1631
• Main Authors: SOON DONG LEE, DONG WAN LEE, KO, Young-Hwan
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.07.2011
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; Fatty Acids - chemistry; Fundamental and applied biological sciences. Psychology; Lipids - analysis; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids - chemistry; Phylogeny; Pigmentation; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Systematics; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Volcanic Eruptions; Republic of Korea; Taxonomy; Bacteria; New species
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.025460-0

A novel actinomycete, designated strain Sco-A36(T), was isolated from volcanic ash. Cells were aerobic, Gram-stain-positive, oxidase-negative, catalase-positive, non-motile cocci; colonies were yellow-coloured, smooth, entire and convex. Phylogenetic analysis showed that the organism formed a distinct phyletic line within the radiation of the genus Marmoricola. Its closest phylogenetic neighbours were Marmoricola aurantiacus DSM 12652(T) (97.9 % 16S rRNA gene sequence similarity), Marmoricola scoriae Sco-D01(T) (97.9 %), Marmoricola aequoreus SST-45(T) (97.4 %) and Marmoricola bigeumensis MSL-05(T) (96.3 %). The diagnostic diamino acid in the cell walls was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H(4)). The polar lipids contained phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The predominant fatty acids were C(16 : 0), C(17 : 1)ω8c, C(18 : 1)ω9c and C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH. The DNA G+C content of strain Sco-A36(T) was 71.0 mol%. Levels of DNA-DNA relatedness between the new isolate and the type strains of recognized species of the genus Marmoricola were 4.9-29.2 %. The phenotypic and DNA-DNA hybridization data presented here strongly suggest that strain Sco-A36(T) represents a novel species of the genus Marmoricola, for which the name Marmoricola korecus sp. nov. is proposed. The type strain is Sco-A36(T) ( = KCTC 19596(T) = DSM 22128(T)).