Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 78; p. 101690
• Main Authors: Manivannan, Kavitha, Mahmoud, Samar Mohamed, Ramasamy, Malathi, Shehata, Abeer A.E., Ahmed, Hanaa, Solaimuthu, Chandrasekar, Dhandapani, Kaviyarasan
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2021; Elsevier Science Ltd
• Subjects: Brucella; Brucella spp; Brucellosis; Camelidae; Camels; Camelus dromedarius; Developing countries; Dromedary camels; Health risks; Kidneys; LDCs; Liver; Livestock; Meat; Real time; Real-time PCR; Spleen; Tissues; Qatar; Dromedary camels; Brucellosis; Brucella spp; Real-time PCR
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2021.101690

•Livestock brucellosis is deliberated as the serious zoonotic disease with abortion, new-born mortality, and infertility.•RT-PCR technique has been reported as a good tool to detect the Brucellosis than conventional PCR with increased performance.•Our findings from this study unveiled the presence of Brucella spp in the dromedary camels of Qatar. Brucellosis in livestock causes huge economic loses of developing countries and demonstrates a serious health risks to the patrons of infected dairy goods and meat. Prevention of brucellosis in cattle relies on the trustworthiness of the techniques utilized to detect the causative pathogen. In the current investigation, we described the exploration of the well-known real-time PCR technique based on the Brucella-specific IS711 with different tissue samples of dromedary camels in Qatar. The findings of the real-time PCR unveiled the occurrence of Brucella spp in 60 % of lung tissues, 71.42 % of liver tissues, 72 % of spleen tissues, 25 % of kidney tissues, and 42.42 % of placental fluid samples. Among them, the liver tissues and spleen tissues possessed the highest number of positive results, whereas kidney tissues displayed the lowest number of positive results for Brucella spp. The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.