Structure and variability of nuclear ribosomal genes in the genus Helianthus

The restriction map of the rDNA unit of Helianthus annuus was constructed using EcoRI, BamHI, HindIII, KpnI and SacI restriction enzymes. Variations in this map among 61 ecotypes representing 39 species of the genus Helianthus were analyzed. The sizes of the rDNA unit ranged from 9.8 to 11.0 kbp, du...

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Published inTheoretical and applied genetics Vol. 76; no. 4; p. 481
Main Authors Choumane, W. (Universite Claude Bernard-Lyon, Villeurbanne (France). 1. Lab. de Biologie Cellulaire associe au CNRS), Heizmann, P
Format Journal Article
LanguageEnglish
Published Germany 01.10.1988
Subjects
CITOLOGIA
CODE GENETIQUE
CODIGO GENETICO
CYTOLOGIE
CYTOLOGY
GENE
GENES
GENETIC CODE
HELIANTHUS
RIBOSOMAS
RIBOSOME
RIBOSOMES
Taxonomie
Vererbung
Zellbestandteil
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Summary:The restriction map of the rDNA unit of Helianthus annuus was constructed using EcoRI, BamHI, HindIII, KpnI and SacI restriction enzymes. Variations in this map among 61 ecotypes representing 39 species of the genus Helianthus were analyzed. The sizes of the rDNA unit ranged from 9.8 to 11.0 kbp, due to a length-repeat heterogeneity of the external non-transcribed spacer by increments of 200 base pair segments. Lengthrepeat heterogeneity and restriction polymorphism were found to be characteristic of populations or species of Helianthus. Restriction patterns and thermal melting with probes of a cloned H. annuus ENTS segment allowed us to differentiate species from each other. However, most lines of the cultivated sunflower were found to be identical on the basis of the physical properties of their ribosomal DNA.
Bibliography:F30
89B0031
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00260896