Structure and variability of nuclear ribosomal genes in the genus Helianthus
The restriction map of the rDNA unit of Helianthus annuus was constructed using EcoRI, BamHI, HindIII, KpnI and SacI restriction enzymes. Variations in this map among 61 ecotypes representing 39 species of the genus Helianthus were analyzed. The sizes of the rDNA unit ranged from 9.8 to 11.0 kbp, du...
Saved in:
Published in | Theoretical and applied genetics Vol. 76; no. 4; p. 481 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Germany
01.10.1988
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Summary: | The restriction map of the rDNA unit of Helianthus annuus was constructed using EcoRI, BamHI, HindIII, KpnI and SacI restriction enzymes. Variations in this map among 61 ecotypes representing 39 species of the genus Helianthus were analyzed. The sizes of the rDNA unit ranged from 9.8 to 11.0 kbp, due to a length-repeat heterogeneity of the external non-transcribed spacer by increments of 200 base pair segments. Lengthrepeat heterogeneity and restriction polymorphism were found to be characteristic of populations or species of Helianthus. Restriction patterns and thermal melting with probes of a cloned H. annuus ENTS segment allowed us to differentiate species from each other. However, most lines of the cultivated sunflower were found to be identical on the basis of the physical properties of their ribosomal DNA. |
---|---|
Bibliography: | F30 89B0031 |
ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/BF00260896 |