A brief introduction to capillary electrophoresis

The present chapter aims to summarize the basic instrumental aspects and separation principles of capillary electrophoresis, with particular attention to those relevant in forensic sciences, and to make comprehensible also to newcomers the following papers of the present monographic volume. In brief...

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Bibliographic Details
Published inForensic science international Vol. 92; no. 2; pp. 75 - 88
Main Authors Tagliaro, F, Manetto, G, Crivellente, F, Smith, F.P
Format Journal Article Conference Proceeding
LanguageEnglish
Published Kidlington Elsevier Ireland Ltd 05.04.1998
Elsevier
Elsevier Limited
Subjects
Analytical chemistry
Blood vessels
Capillary electrophoresis
Capillary zone
CE-LIF
CE-UV
CEC
CE–MS
CGE
Chemistry
Chromatographic methods and physical methods associated with chromatography
CIEF
CITP
CZE
Electrochromatography
Electrodes
Electrophoresis
Exact sciences and technology
Forensic science
Forensic sciences
High voltage
Injection systems
Isoelectric focusing
Isotachophoresis
MECC
Other chromatographic methods
Reproducibility
Sensors
Separation
Separation techniques
Viscosity
CGE
CEC
CE–MS
Capillary electrophoresis
CIEF
CE-LIF
MECC
CE-UV
CITP
CZE
Capillary column
Separation method
Instrumentation
Zone electrophoresis
Analysis method
Review
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Summary:The present chapter aims to summarize the basic instrumental aspects and separation principles of capillary electrophoresis, with particular attention to those relevant in forensic sciences, and to make comprehensible also to newcomers the following papers of the present monographic volume. In brief, a capillary electropherograph consists of an injection system, a separation capillary (20–100 μm I.D., 20–100 cm length), a high voltage source (delivering up to 30 kV and up to 200–250 μA), electrodes and electrode jars and detector(s). All these instrumental components are described, with emphasis on the detection techniques. In addition, the main separation techniques are presented, including capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary electrochromatography, capillary isotachophoresis, capillary gel electrophoresis, capillary isoelectric focusing and chiral separation methods.
ISSN:0379-0738
1872-6283
DOI:10.1016/S0379-0738(98)00010-3