Antibacterial Activity of Tanacetum vulgare L. Extracts against Clinical Isolates of Bovine Mastitis

• Published in: Applied sciences Vol. 13; no. 5; p. 3369
• Main Authors: Šukele, Renāte, Bārzdiņa, Ance, Koka, Rudīte, Skadins, Ingus, Lauberte, Līga, Brangule, Agnese, Kovalcuka, Liga, Bandere, Dace
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.03.2023
• Subjects: Antibacterial activity; Antibiotics; Antimicrobial agents; Bacteria; bovine mastitis; Cattle; Chemical compounds; Clinical isolates; E coli; Escherichia coli; Ethanol; Flowers; Flowers & plants; Herbal medicine; Infectious diseases; Leaves; Mastitis; minimal bactericidal concentration; Minimum inhibitory concentration; Oils & fats; Phenolic compounds; Phenols; Plant extracts; Serratia liquefaciens; Solvents; Staphylococcus aureus; Strains (organisms); Streptococcus agalactiae; Streptococcus uberis; Tanacetum vulgare; United Kingdom > UK; Latvia; Germany
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app13053369

A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternatively, herbal medicine has been proposed due to bacterial resistance. The aim of this study was to determine the antibacterial activity of the acetonic and ethanolic extracts of dried flowers and leaves of Tanacetum vulgare L. against bovine mastitis-inducing clinical isolates such as Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and reference cultures of S. aureus and E. coli. The extracts of T. vulgare showed partial antibacterial activity against tested strains of S. aureus. The MIC and MBC values of a 70% ethanol extract of flowers (MIC = 3.4 mg/mL, MBC = 3.4–6.8 mg/mL) were lower than for the 70% ethanol extract of leaves (MIC = 15.7–31.4 mg/mL, MBC = 62.9–125.9 mg/mL). The flower extracts showed low activity against E. coli (MIC = 53.9 mg/mL, MBC = 53.9–107.8 mg/mL) and S. agalactiae (MIC, MBC = 53.9 mg/mL). T. vulgare leaf extracts had minimal antibacterial effects against Streptococcus strains (MIC = 31.4–62.9 mg/mL, MBC = 53.9–125.9 mg/mL) and Serratia liquefaciens (MIC, MBC = 125.9 mg/mL). However, flower extracts had a higher phenolic content that did not correlate with antibacterial effects. T. vulgare flower and leaf extracts could be combined to obtain broader antibacterial effects.