Design of Inverted Nano-Cone Arrayed SERS Substrate for Rapid Detection of Pathogens

Rapid detection of bacteria is a very critical and important part of infectious disease treatment. Sepsis kills more than 25 percent of its victims, resulting in as many as half of all deaths in hospitals before identifying the pathogen for patients to get the right treatment. Raman spectroscopy is...

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Bibliographic Details
Published inApplied sciences Vol. 11; no. 17; p. 8067
Main Authors Jia, Zixun, Asiri, Sarah, Elsharif, Asma, Alamoudi, Widyan, Al-Suhaimi, Ebtesam, Kim, Sang-Gook
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.09.2021
Subjects
Atoms & subatomic particles
Bacteria
Bacterial infections
Biosensors
Cones
Design
Electric fields
Geometry
gold nanorods
Health services
Infectious diseases
Light
Magnetic fields
Medical treatment
nanocone array
Nanoparticles
Numerical analysis
pathogen detection
Pathogens
Photons
Polydimethylsiloxane
Raman spectroscopy
Sensitivity
Sepsis
SERS
Simulation
Spectroscopy
Spectrum analysis
Substrates
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Summary:Rapid detection of bacteria is a very critical and important part of infectious disease treatment. Sepsis kills more than 25 percent of its victims, resulting in as many as half of all deaths in hospitals before identifying the pathogen for patients to get the right treatment. Raman spectroscopy is a promising candidate in pathogen diagnosis given its fast and label-free nature, only if the concentration of the pathogen is high enough to provide reasonable sensitivity. This work reports a new design of surface-enhanced Raman spectroscopy (SERS) substrate which will provide high enough sensitivity and fast and close contact of the target structure to the optical hot spots for immunomagnetic capturing-based bacteria-concentrating technique. The substrate uses inverted nanocone structure arrays made of transparent PDMS (Polydimethylsiloxane) to funnel the light from the bottom to the top of the cones where plasmonic gold nanorods are located. A high reflective and low loss layer is deposited on the outer surface of the cone. Given the geometry of cones, photons are multi-reflected by the outer layer and thus the number density of photons at hotspots increases by an order of magnitude, which could be high enough to detect immunomagnetically densified bacteria.
ISSN:2076-3417
2076-3417
DOI:10.3390/app11178067