Leucine-rich repeat-containing G protein-coupled receptor 5 expression in lymph node metastases of colorectal cancer: Clinicopathological insights and prognostic implications

• Published in: Pathology international Vol. 74; no. 7; pp. 387 - 393
• Main Authors: Sawaguchi, Hiroshi, Uehara, Takeshi, Iwaya, Mai, Asaka, Shiho, Nakajima, Tomoyuki, Kamakura, Masato, Nagaya, Tadanobu, Yoshizawa, Takahiro, Ota, Hiroyoshi, Umemura, Takeji
• Format: Journal Article
• Language: English
• Published: Australia Wiley Subscription Services, Inc 01.07.2024; John Wiley and Sons Inc
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor - analysis; Biomarkers, Tumor - metabolism; Cancer; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Female; Gene expression; Humans; Leucine; Lymph nodes; Lymph Nodes - metabolism; Lymph Nodes - pathology; Lymphatic Metastasis - pathology; Lymphatic system; Male; Metastases; Metastasis; Middle Aged; Necrosis; Original; Prognosis; Proteins; Receptors; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; Stem cells; Surgical equipment; Tumor cells; Tumors; colorectal cancerl; RNA in situ hybridization; eucine‐rich repeat‐containing G‐protein‐coupled receptor 5; lymph node metastasis
• ISSN: 1320-5463; 1440-1827; 1440-1827
• DOI: 10.1111/pin.13439

Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), a significant cancer stem cell marker in colorectal cancer (CRC), lacks lymph node (LN) expression studies. In this study, we identified LGR5 expression by RNAscope, a highly sensitive RNA in situ method, and analyzed its association with clinicopathological characteristics. Tissue microarrays were generated from primary tumors (PTs) and LN metastases in paraffin-embedded blocks of 38 CRC surgical resection materials. LGR5 expression by RNAscope was evaluated by dividing the expression levels into negative and positive expression. In all but two cases of LN metastasis, LGR5-positive dots were detected in tumor cells, and there was a wide range of LGR5-positive cells. More LGR5-positive dots were identified in the gland-forming region. Twenty-three cases were classified into a high LGR5-expression group, and 15 cases were classified into a low LGR5-expression group. In the high LGR5-expression group, the histological grade was lower than in the low LGR5-expression group (p = 0.0159), while necrosis was significantly more prevalent (p = 0.0326), and the tumor, node, metastasis stage was significantly lower (p = 0.0302). There was no association between LGR5 expression levels in LN metastases and LGR5 expression levels in PT tissue. LGR5 expression in LN metastases may influence prognosis. Further analysis may lead to new therapeutic strategies.