Plasmids in Listeria

Thirty-two Listeria strains, including L. monocytogenes of the different Seeliger serovars, L. grayi and L. murrrayi , have been analyzed for the presence of plasmids. A cryptic plasmid of 38.5 Md was found in seven strains: in four L. monocytogenes (serovars 1/2c, 4b, 4d, and Ivanov 1), and in both...

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Bibliographic Details
Published inPlasmid Vol. 8; no. 2; pp. 112 - 118
Main Authors Pérez-Díaz, J C, Vicente, M F, Baquero, F
Format Journal Article
LanguageEnglish
Published United States 01.01.1982
Subjects
DNA, Bacterial - analysis
DNA, Circular - analysis
Listeria
Listeria - genetics
Listeria grayi
Listeria monocytogenes
Plasmids
Streptococcus
Streptococcus agalactiae
Streptococcus agalactiae - genetics
Transformation, Genetic
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Summary:Thirty-two Listeria strains, including L. monocytogenes of the different Seeliger serovars, L. grayi and L. murrrayi , have been analyzed for the presence of plasmids. A cryptic plasmid of 38.5 Md was found in seven strains: in four L. monocytogenes (serovars 1/2c, 4b, 4d, and Ivanov 1), and in both the L. grayi and the single L. murrayi strains. Restriction enzyme cleavage analysis with two restriction enzymes suggests that the plasmid is a single molecular species, pRYC16. Conjugation experiments between Streptococcus agalactiae BM6101 containing the resistance plasmid pIP501 and L. monocytogenes, L. garayi , or L. murrayi demonstrated that pIP501 is transferred to Listeria at a mean frequency of 10 super(-6). pIP501 is fully expressed in Listeria and promotes its own transfer between strains of Listeria and from Listeria back to Streptococcus . It is suggested that a circulation of plasmids can take place among members of the genera Listeria and Streptococcus .
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ISSN:0147-619X
DOI:10.1016/0147-619X(82)90049-X