Effects of Microbial Inoculants on the Fermentation and Preservation of Triticale Silages at High and Low Moisture Levels

• Published in: Applied sciences Vol. 10; no. 21; p. 7855
• Main Authors: Soundharrajan, Ilavenil, Muthusamy, Karnan, Han, Ouk-Kyu, Lee, Hyun Jeong, Purushothaman, Sumitha, Kim, Dahye, Choi, Ki Choon
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.11.2020
• Subjects: Acetic acid; Antibacterial activity; Bacteria; Biological activity; Butyric acid; Carbohydrates; Dry matter; Fermentation; L. paracasei; L. rhamnosus; Laboratories; Lactic acid; Lactobacilli; low and high moistures; Metabolites; Microorganisms; Milk; Moisture; Nutrients; Probiotics; Silage; Triticale; triticale silage; Yeasts
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app10217855

In the current study, Lactobacillus rhamnosus (KCC-51) and Lactobacillus paracasei (KCC-52) were isolated and investigated for their biological potential including antibacterial activity, probiotic potential, nutritional changes, fermentation ability, and microbial population in experimental silages at high moisture (HM) and low moisture (LM) conditions. These strains showed significant antibacterial activity and probiotic activities. There are no significant changes in the dry matter content (DM) and nutritional profiles of control, KCC-51, and KCC-52 treated silages. High lactic acid (LA) and low butyric acid (BA) levels were noted in the HM (LA: 4.82 ± 0.34 and 5.5 ± 0.03, BA: 0.01 ± 0.01 and 0.008 ± 0.001, %DM) and LM (LA: 2.27 ± 0.32 and 2.61 ± 0.2, BA: 0.017 ± 0.001 vs. 0.006 ± 0.05, %DM) silages treated with KCC-51 and KCC-52 respectively, compared to non-inoculated silages. KCC-51 and KCC-52 treatment increased acceptable range of acetic acid (AA) in HM (0.33 ± 0.02 and 0.24 ± 0.007, %DM) and LM (0.22 ± 0.007 and 0.35 ± 0.02, %DM) silages compared to non-inoculated silages (0.22 ± 0.03 and 0.17 ± 0.05, %DM). Rich LAB and low yeast counts were noted in the silages treated with KCC-51 and KCC-52, it indicated that the addition of these inoculants strongly dominated the enterobacterial growth and preserved the silage quality with essential metabolites. It suggests that L. rhamnosus and the L. paracasei could be used as potent inoculants for the silage production with enriched nutrients.