Whole genome sequencing and comparative genomics of Mycobacterium orygis isolated from different animal hosts to identify specific diagnostic markers
, a member of MTBC has been identified in higher numbers in the recent years from animals of South Asia. Comparative genomics of this important zoonotic pathogen is not available which can provide data on the molecular difference between other MTBC members. Hence, the present study was carried out t...
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Published in | Frontiers in cellular and infection microbiology Vol. 13; p. 1302393 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
2023
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Subjects | |
Online Access | Get full text |
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Summary: | , a member of MTBC has been identified in higher numbers in the recent years from animals of South Asia. Comparative genomics of this important zoonotic pathogen is not available which can provide data on the molecular difference between other MTBC members. Hence, the present study was carried out to isolate, whole genome sequence
from different animal species (cattle, buffalo and deer) and to identify molecular marker for the differentiation of
from other MTBC members.
Isolation and whole genome sequencing of
was carried out for 9 samples (4 cattle, 4 deer and 1 buffalo) died due to tuberculosis. Comparative genomics employing 53 genomes (44 from database and 9 newly sequenced) was performed to identify SNPs, spoligotype, pangenome structure, and region of difference.
was isolated from water buffalo and sambar deer which is the first of its kind report worldwide. Comparative pangenomics of all
strains worldwide (n= 53) showed a closed pangenome structure which is also reported for the first time. Pairwise SNP between TANUVAS_2, TANUVAS_4, TANUVAS_5, TANUVAS_7 and NIRTAH144 was less than 15 indicating that the same
strain may be the cause for infection. Region of difference prediction showed absence of RD7, RD8, RD9, RD10, RD12, RD301, RD315 in all the
analyzed. SNPs in virulence gene, PE35 was found to be unique to
which can be used as marker for identification.
The present study is yet another supportive evidence that
is more prevalent among animals in South Asia and the zoonotic potential of this organism needs to be evaluated. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2235-2988 2235-2988 |
DOI: | 10.3389/fcimb.2023.1302393 |