Physical map of chloroplast DNA in sugi, Cryptomeria japonica

To investigate the evolution of conifer species, a physical map of the chloroplast DNA of sugi, Cryptomeria japonica was constructed, with four restriction endonucleases, Pst1, Sal1, Sac1 and Xho1. The chloroplast genome of C. japonica was found to be a circular molecule with a total size of approxi...

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Published inTheoretical and applied genetics Vol. 86; no. 2-3; p. 166
Main Authors Tsumura, Y. (Forestry and Forest Products Research Inst. Kukizaki, Ibaraki (Japan). Bio-resource Technology Div.), Ogihara, Y, Sasakuma, T, Ohba, K
Format Journal Article
LanguageEnglish
Published Germany 01.04.1993
Subjects
ADN
CARTE GENETIQUE
Chloroplast DNA
CHLOROPLASTE
CLOROPLASTO
CRYPTOMERIA JAPONICA
EVOLUCION
EVOLUTION
Evolution of conifers
MAPAS GENETICOS
Physical map
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Summary:To investigate the evolution of conifer species, a physical map of the chloroplast DNA of sugi, Cryptomeria japonica was constructed, with four restriction endonucleases, Pst1, Sal1, Sac1 and Xho1. The chloroplast genome of C. japonica was found to be a circular molecule with a total size of approximately 133 kb. This molecule lacked an inverted repeat. Twenty genes were localized on the physical map of C. japonica cpDNA by Southern hybridization. The chloroplast genome structure of C. japonica showed considerable rearrangements of the standard genome type found in vascular plants and differed markedly from that of tobacco. The difference was explicable by one deletion and five inversions. The chloroplast genome of C. japonica differed too from that of the genus Pinus which also lacks one of the inverted repeats. The results indicate that the conifer group originated monophyletically from an ancient lineage, and diverged independently after loss of an inverted repeat structure
Bibliography:F30
93B0591
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00222075