Soy isoflavones administered to rats from weaning until sexual maturity affect ovarian follicle development by inducing apoptosis

• Published in: Food and chemical toxicology Vol. 72; pp. 51 - 60
• Main Authors: Wang, Wenxiang, Sun, Yan, Liu, Jin, Li, Yuchen, Li, Hong, Xiao, Shihua, Weng, Shaozheng, Zhang, Wenchang
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier 01.10.2014
• Subjects: Animals; Apoptosis - drug effects; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Biological and medical sciences; Caspase 3 - genetics; Caspase 3 - metabolism; Caspase 8 - genetics; Caspase 8 - metabolism; Caspase 9 - genetics; Caspase 9 - metabolism; Dose-Response Relationship, Drug; fas Receptor - genetics; fas Receptor - metabolism; Female; Food toxicology; Genistein - blood; Granulosa Cells - drug effects; Granulosa Cells - ultrastructure; In Situ Nick-End Labeling; Isoflavones - blood; Isoflavones - pharmacology; Medical sciences; Microscopy, Electron, Transmission; Rats; Rats, Wistar; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal Transduction; Soy Foods - analysis; Toxicology; Weaning; Isoflavone; Phytoestrogens; Rat; Weaning; Rodentia; Follicle; Soybean; Isoflavone derivatives; Phytoestrogen; Grain legume; Ovary; Vertebrata; Sexual maturity; Mammalia; Soy isoflavones; Cell death; Animal; Development; Ovarian follicle; Apoptosis
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2014.07.010

Twenty-one-day-old female Wistar rats were treated daily with orally administered soy isoflavones (SIFs) at concentrations of 50, 100, or 200 mg/kg body weight from weaning until sexual maturity (3 mo.), and ovarian follicle development was evaluated. At the end of the treatment period, the ultrastructure of the ovarian granulosa cells was examined by transmission electron microscopy. The apoptotic cell death of ovarian granulosa cells was detected using TUNEL staining. The mRNA expression levels of caspase-3, caspase-8, caspase-9, Bcl2, Bax, and Fas were determined by real-time quantitative PCR. The protein expression levels of caspase-3, Bcl2, Bax, and Fas were determined by western blotting. Our data showed that exposure to SIFs resulted in morphological changes consistent with ovarian granulosa cell apoptosis. The percentage of TUNEL-positive granulosa cells was increased. The mRNA expression levels of the apoptosis-related genes caspase-3, caspase-8, caspase-9, Bax, and Fas increased significantly. The protein levels of Bax, Fas, and cleaved caspase-3 were also increased. These results indicate that the exposure of rats to modest doses of SIFs from weaning until sexual maturity can affect ovarian follicle development by inducing apoptosis. The mechanism of SIF-induced alterations in ovarian follicle development may involve the activation of Fas-mediated and Bcl2/Bax-mediated apoptotic signaling pathways.