Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 6; no. 1; pp. 7 - 19
• Main Authors: Kim, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), Kim, S.H. (Seoul National University, Seoul, Republic of Korea), Kwon, N.H. (Seoul National University, Seoul, Republic of Korea), Bae, W.K. (Seoul National University, Seoul, Republic of Korea), Lim, J.Y. (Seoul National University, Seoul, Republic of Korea), Koo, H.C. (Seoul National University, Seoul, Republic of Korea), Kim, J.M. (Seoul National University, Seoul, Republic of Korea), Noh, K.M. (Seoul National University, Seoul, Republic of Korea), Jung, W.K. (Seoul National University, Seoul, Republic of Korea), Park, K.T. (Seoul National University, Seoul, Republic of Korea), Park, Y.H. (Seoul National University, Seoul, Republic of Korea), E-mail: yhp@snu.ac.kr
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 01.03.2005
• Subjects: Abattoirs; Adhesins, Bacterial; Adhesins, Bacterial - genetics; Animals; Cattle; Cercopithecus aethiops; Chickens; Chlorocebus aethiops; ESCHERICHIA COLI; Escherichia coli O157; Escherichia coli O157 - genetics; Escherichia coli O157 - isolation & purification; Escherichia coli Proteins; Escherichia coli Proteins - genetics; Feces; Feces - microbiology; Food Microbiology; food pathogens; genetics; Hemolysin Proteins; Hemolysin Proteins - genetics; isolation & purification; Korea; Meat; Meat - microbiology; methods; microbiology; PCR; Phylogeny; Polymerase Chain Reaction; Polymerase Chain Reaction - methods; random amplified polymorphic DNA technique; Random Amplified Polymorphic DNA Technique - methods; RAPD; rapid methods; Reagent Kits, Diagnostic; Shiga Toxin 1; Shiga Toxin 1 - genetics; Shiga Toxin 2; Shiga Toxin 2 - genetics; South Korea; Swine; United States; Vero Cells; 수의학; United States; Korea; South Korea; multiplex PCR; RAPD; Escherichia coli O157:H7
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2005.6.1.7

Escherichia coli O157:H7 is recognized as a significant food-borne pathogen, so rapid identification is important for food hygiene management and prompt epidemiological investigations. The limited prevalence data on Shiga toxin-producing E. coli (STEC) and E. coli O157:H7 in foods and animals in Korea made an assessment of the risks difficult, and the options for management and control unclear. The prevalence of the organisms was examined by newly developed kit-E. coli O157:H7 Rapid kit. For the isolation of E. coli O157:H7, conventional culture, immunomagnetic separation, and E. coli O157:H7 Rapid kit were applied, and multiplex PCR and randomly amplified polymorphic DNA (RAPD) were performed for the molecular determination. There was high molecular relatedness among 11 Korean isolates and 17 U.S. strains at 63% level.