Reticine A, a new potent natural elicitor: isolation from the fruit peel of Citrus reticulate and induction of systemic resistance against tobacco mosaic virus and other plant fungal diseases

• Published in: Pest management science Vol. 77; no. 1; pp. 354 - 364
• Main Authors: Wang, Delong, Liu, Bin, Ma, Zhiqing, Feng, Juntao, Yan, He
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.01.2021; Wiley Subscription Services, Inc
• Subjects: Biocides; Biosynthesis; Citrus; Citrus fruits; Citrus reticulata; Citrus reticulate peel; Disease resistance; Enzymatic activity; Fruit; Fruits; Fungal diseases; Gene expression; Genes; Humans; Hydrogen peroxide; Lead compounds; Mycoses; Nicotiana; Optimization; Pathogenesis; Pest control; Pest resistance; Plant Diseases; Plant extracts; Plant Proteins; reticine A; Salicylic acid; Salicylic Acid - pharmacology; systemic acquired resistance; Tobacco; Tobacco Mosaic Virus; Tobamovirus; Virulence; Viruses; systemic acquired resistance; Citrus reticulate peel; reticine A
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.6025

Background Systemic acquired resistance (SAR) induced by elicitors is a highly satisfying form of resistance that protects plants against invading pathogens. Exploration and development of new elicitors is a promising alternative to conventional biocides in resistant pest management. In our previous broad screening, fruit peel extract of Citrus reticulata Blanco exhibited the ability to induce SAR in tobacco. Result A new potent elicitor reticine A was isolated from the fruit peel extract of industrial crop C. reticulate and its structure was well elucidated. In vivo assays showed that reticine A had considerable control efficacies at 100 and 500 μg mL−1, being superior to commercial elicitor benzothiadiazole (BTH) (100 μg mL−1). Reticine A had no significant impact on the virulence of tobacco mosaic virus (TMV) particles under in vitro conditions. Application of reticine A induced a local hypersensitive reaction (HR), systemic accumulation of H2O2 and salicylic acid (SA), systemic increase in defensive enzyme activities and systemic upregulated expression of pathogenesis‐related (PR) proteins, suggesting its induction of SAR in tobacco. The expression of NPR1 and SA biosynthesis genes ICS and PAL were systemically upregulated. Conclusion SAR induced by reticine A against TMV in tobacco was demonstrated and the mechanism might be attributed to activating the expression of several defensive genes mediated by an SA signal. This study highlights the potential of reticine A which is recommended to be applied directly or as an active ingredient in the crude extract formulation ahead of time in the field, as well as being a potential lead compound for further optimization. A new potent natural elicitor reticine A was isolated from the fruit peel extract of Citrus reticulate. Systemic acquired resistance induced by reticine A against tobacco mosaic virus (TMV) in tobacco was demonstrated. © 2020 Society of Chemical Industry