Capillary blood self‐collection for high‐throughput proteomics

• Published in: Proteomics (Weinheim) Vol. 24; no. 16; pp. e2300607 - n/a
• Main Authors: El‐Sabawi, Bassim, Huang, Shi, Tanriverdi, Kahraman, Perry, Andrew S., Amancherla, Kaushik, Jackson, Natalie, Hulsey, Jenna, Freedman, Jane E., Shah, Ravi, Lindman, Brian R.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.08.2024
• Subjects: Adult; Blood; blood proteins; Blood Proteins - analysis; blood specimen collection; Blood Specimen Collection - methods; Coefficient of variation; Collection; Correlation; Female; High-Throughput Screening Assays - methods; Humans; Male; Middle Aged; Phlebotomy; Phlebotomy - methods; Proteins; Proteomics; Proteomics - methods; tasso; blood specimen collection; tasso; blood proteins; proteomics
• ISSN: 1615-9853; 1615-9861; 1615-9861
• DOI: 10.1002/pmic.202300607

In this study, we sought to compare protein concentrations obtained from a high‐throughput proteomics platform (Olink) on samples collected using capillary blood self‐collection (with the Tasso+ device) versus standard venipuncture (control). Blood collection was performed on 20 volunteers, including one sample obtained via venipuncture and two via capillary blood using the Tasso+ device. Tasso+ samples were stored at 2°C–8°C for 24‐hs (Tasso‐24) or 48‐h (Tasso‐48) prior to processing to simulate shipping times from a study participant's home. Proteomics were analyzed using Olink (384 Inflammatory Panel). Tasso+ blood collection was successful in 37/40 attempts. Of 230 proteins included in our analysis, Pearson correlations (r) and mean coefficient of variation (CV) between Tasso‐24 or Tasso‐48 versus venipuncture were variable. In the Tasso‐24 analysis, 34 proteins (14.8%) had both a correlation r > 0.5 and CV < 0.20. In the Tasso‐48 analysis, 68 proteins (29.6%) had a correlation r > 0.5 and CV < 0.20. Combining the Tasso‐24 and Tasso‐48 analyses, 26 (11.3%) proteins met these thresholds. We concluded that protein concentrations from Tasso+ samples processed 24–48 h after collection demonstrated wide technical variability and variable correlation with a venipuncture gold‐standard. Use of home capillary blood self‐collection for large‐scale proteomics should be limited to select proteins with good agreement with venipuncture.