Zebrafish cardiac enhancer trap lines: New tools for in vivo studies of cardiovascular development and disease

Using the transposon‐mediated enhancer trap (ET), we generated 18 cardiac enhancer trap (CET) transgenic zebrafish lines. They exhibit EGFP expression in defined cell types—the endocardium, myocardium, and epicardium—or in anatomical regions of the heart—the atrium, ventricle, valves, or bulbus arte...

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Published inDevelopmental dynamics Vol. 239; no. 3; pp. 914 - 926
Main Authors Poon, Kar‐Lai, Liebling, Michael, Kondrychyn, Igor, Garcia‐Lecea, Marta, Korzh, Vladimir
Format Journal Article
LanguageEnglish
Published New York Wiley‐Liss, Inc 01.03.2010
Subjects
Animals
atrium
bulbus arteriosus
Cardiovascular Diseases - pathology
Cardiovascular System
Disease Models, Animal
endocardium
Endocardium - pathology
Enhancer Elements, Genetic
epicardium
fast scanning confocal imaging
fhla
Genetic Techniques
Green Fluorescent Proteins - metabolism
Heart - embryology
Heart Atria - pathology
image rendering
Microscopy, Confocal - methods
myocardium
Myocardium - metabolism
Pericardium - pathology
Transgenes
valves
ventricle
Zebrafish
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Summary:Using the transposon‐mediated enhancer trap (ET), we generated 18 cardiac enhancer trap (CET) transgenic zebrafish lines. They exhibit EGFP expression in defined cell types—the endocardium, myocardium, and epicardium—or in anatomical regions of the heart—the atrium, ventricle, valves, or bulbus arteriosus. Most of these expression domains are maintained into adulthood. The genomic locations of the transposon insertions were determined by thermal asymmetric interlaced polymerase chain reaction (TAIL‐PCR). The expression pattern of EGFP in some CETs is unique and recapitulates expression of genes flanking the transposon insertion site. The CETs enabled us to capture the dynamics of the embryonic heart beating in vivo using fast scanning confocal microscopy coupled with image reconstruction, producing three‐dimensional movies in time (4D) illustrating region‐specific features of heart contraction. This collection of CET lines represents a toolbox of markers for in vivo studies of heart development, physiology, and drug screening. Developmental Dynamics 239:914–926, 2010. © 2010 Wiley‐Liss, Inc.
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content type line 23
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.22203