Inhibitory effect of resveratrol on angiotensin II-induced cardiomyocyte hypertrophy

• Published in: Naunyn-Schmiedeberg's archives of pharmacology Vol. 369; no. 2; pp. 239 - 244
• Main Authors: Cheng, Tzu-Hurng, Liu, Ju-Chi, Lin, Heng, Shih, Neng-Lang, Chen, Yen-Ling, Huang, Meng-Ting, Chan, Paul, Cheng, Ching-Feng, Chen, Jin-Jer
• Format: Journal Article
• Language: English
• Published: Germany 01.02.2004
• Subjects: Acetylcysteine - pharmacology; Angiotensin II - metabolism; Angiotensin II - pharmacology; Animals; Animals, Newborn; Antioxidants - pharmacology; Cells, Cultured; Hypertrophy - prevention & control; Leucine - metabolism; Mitogen-Activated Protein Kinases - metabolism; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - pathology; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; Myosin Heavy Chains - genetics; Phosphorylation; Promoter Regions, Genetic; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Stilbenes - pharmacology; Transfection; Ventricular Myosins - genetics
• ISSN: 0028-1298; 1432-1912
• DOI: 10.1007/s00210-003-0849-6

Resveratrol is proposed to account in part for the protective effect of red wine on the cardiovascular system. Angiotensin II (Ang II) is a potent hypertrophic stimulus in cardiomyocytes. In this study, we determined the effect of resveratrol on Ang II-induced cardiomyocyte hypertrophy. Cultured neonatal rat cardiomyocytes were stimulated with Ang II, and [3H]leucine incorporation and beta-myosin heavy chain (beta-MyHC) promoter activity were examined. Intracellular reactive oxygen species (ROS) were measured by a redox-sensitive fluorescent dye, 2' 7'-dichlorofluorescin diacetate, and the extracellular signal-regulated kinase (ERK) phosphorylation was examined by Western blotting. Resveratrol inhibited Ang II-increased intracellular ROS levels. Furthermore, resveratrol, as well as the antioxidant N-acetyl-cysteine, decreased Ang II- or H2O2-increased protein synthesis, beta-MyHC promoter activity, and ERK phosphorylation. In summary, we demonstrate for the first time that resveratrol inhibits Ang II-induced cardiomyocyte hypertrophy via attenuation of ROS generation.