Differential Effects of N- and C-Terminal Deletions on the Two Activities of Rubisco Activase

• Published in: Archives of biochemistry and biophysics Vol. 326; no. 1; pp. 100 - 105
• Main Authors: Esau, Brian D., Snyder, Gordon W., Portis, Jr, Archie R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.1996
• Subjects: Amino Acid Sequence; ATP hydrolysis; DNA, Complementary - genetics; DNA, Recombinant - genetics; enzyme regulation; Escherichia coli - enzymology; Escherichia coli - genetics; Gene Deletion; Molecular Sequence Data; Mutagenesis, Site-Directed; photosynthesis; Plant Proteins; recombinant DNA; ribulose-bisphosphate carboxylase; Ribulose-Bisphosphate Carboxylase - genetics; Ribulose-Bisphosphate Carboxylase - metabolism; rubisco; Sequence Alignment; Spinacia oleracea - enzymology; recombinant DNA; ATP hydrolysis; photosynthesis; enzyme regulation; rubisco
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1006/abbi.1996.0052

Spinach (Spinacea oleracea) leaf ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase was subjected to limited proteolysis with trypsin and directed deletions were made by modifying the spinach rubisco activase cDNA and expressing the 41-kDa isoform inEscherichia coli.Protein exposed to trypsin displayed a more rapid loss of the ability to promote the activation of decarbamylated rubisco than ATP hydrolysis (e.g., 10 and 50% activity remaining, respectively, after 1 h). A series of N-terminal deletions exhibited near abolition of rubisco activation after the 12th residue, a conserved tryptophan, was deleted. Conversely, a deletion of 19 residues at the C-terminus increased rubisco activation with little effect on ATP hydrolysis, resulting in an increased efficiency of activation. The C-terminal deletion mutant was further modified by a site-directed mutation in the ATP binding region (Q109E) which was previously observed to increase the efficiency of activation (J. B. Shen and W. L. Ogren, 1991,Plant Physiol.99, 1201–1207). The efficiency of activation with this double mutant was greater than that for either of the original mutants. The results indicate that a conserved tryptophan in the N-terminal portion of rubisco activase is critical for promotion of the activation of rubisco, consistent with a possible role in interaction with rubisco. The C-terminus appears to have a regulatory effect on both rubisco activation and ATP hydrolysis.