Preparation of n-3 PUFAs ethyl esters by an efficient biocatalyzed solvent-free process

• Published in: Journal of molecular catalysis. B, Enzymatic Vol. 84; pp. 173 - 176
• Main Authors: Morrone, R., D’Antona, N., Biondi, D., Lambusta, D., Nicolosi, G.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.2012; Elsevier
• Subjects: alpha-linolenic acid; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; catalytic activity; Diethyl carbonate; docosahexaenoic acid; eicosapentaenoic acid; esterification; esters; ethanol; Fundamental and applied biological sciences. Psychology; Irreversible esterification; Lipase; Methods. Procedures. Technologies; omega-3 fatty acids; Pseudozyma antarctica; PUFA; solvents; Triethyl orthoformate; yields; Diethyl carbonate; Irreversible esterification; Lipase; PUFA; Triethyl orthoformate; Biocatalysis; Enzyme; Polyunsaturated fatty acid; Triacylglycerol lipase; Esterases; Esterification; Carboxylic ester hydrolases; Ester; Hydrolases; Carbonates; Operating process; Solvent
• ISSN: 1381-1177; 1873-3158
• DOI: 10.1016/j.molcatb.2012.04.009

[Display omitted] ► Irreversible biocatalyzed esterification of n-3 PUFAs has been obtained by triethyl orthoformate or diethyl carbonate as alcohol donors. ► Preparation in high yields of n-3 PUFA ethyl esters. ► Solvent free biocatalyzed esterification of fatty acids. alpha-Linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been subjected to esterification with ethanol in presence of lipase B from Candida antarctica (Novozym 435®), in solvent free condition. The use of alcohol donors triethyl orthoformate (TEOF) or diethyl carbonate (DEC) instead of free ethanol, allowed working in irreversible esterification conditions and ALA-, EPA- and DHA-ethyl esters were obtained in quantitative yields.