Profiling gibberellin (GA 3)-responsive genes in mature mandarin fruit using a citrus 22K oligoarray

• Published in: Scientia horticulturae Vol. 116; no. 3; pp. 291 - 298
• Main Authors: Fujii, Hiroshi, Shimada, Takehiko, Sugiyama, Aiko, Endo, Tomoko, Nishikawa, Fumie, Nakano, Michiharu, Ikoma, Yoshinori, Shimizu, Tokuro, Omura, Mitsuo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2008; [Amsterdam; New York, NY]: Elsevier Science; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; chloroplasts; Citrus; Citrus unshiu; ethylene; Fundamental and applied biological sciences. Psychology; gene expression; gene expression regulation; genes; Gibberellin; gibberellins; mandarins; Microarray; microarray technology; Non-climacteric; pathogenesis-related proteins; photosynthesis; plant proteins; plant stress; resistance mechanisms; Ripening; transcription (genetics); Citrus; Ripening; Gibberellin; Non-climacteric; Microarray; Horticulture; Citrus fruit; Rutaceae; Gene; Dicotyledones; Angiospermae; Plant growth substance; Spermatophyta; Gibberellic acid; Mandarin; Maturity
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2008.01.010

Gibberellin 3 (GA 3)-responsive genes were investigated with a citrus 22K oligoarray 1 1 The information regarding citrus 22K oligoarray is available at the website http://www.fruit.affrc.go.jp/cgat/. to further the understanding of transcriptional regulation by GA 3 treatment in Satsuma mandarin fruit ( Citrus unshiu Marc.). 213 GA 3-responsive genes were identified that showed a 3-fold or greater expression change after 72 h GA 3 treatment, compared to expression after 72 h air treatment. GA 3 treatment induced expression of pathogenesis-related (PR) proteins and genes that function in photosynthesis, chloroplast biogenesis, resistance, defense and stress. Also, GA 3 treatment reduced the transcription of several ethylene-inducible genes, such as carotenoid metabolic genes, which are associated with fruit ripening. Contrasting effects between GA 3 and ethylene were observed on photosynthesis and chloroplast biogenesis, chlorophyll metabolism, and carotenoid metabolism, indicating that the endogenous GA 3 level might be important for the endogenous regulation of maturation and senescence in mature citrus fruit. It was also found that the GA response pathway was likely to take part in cross-talk with the pathogen-related pathway in mature citrus fruit.