Structure of arabinogalactan from Larix laricina and its reactivity with antibodies directed against type-II-arabinogalactans

• Published in: Carbohydrate polymers Vol. 86; no. 4; pp. 1739 - 1744
• Main Authors: Goellner, Esther Marie, Utermoehlen, Jon, Kramer, Rainer, Classen, Birgit
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.10.2011; Elsevier
• Subjects: Antibodies; Applied sciences; Arabinogalactan; arabinogalactans; cross reaction; dietary fiber; Echinacea purpurea; ELISA; enzyme-linked immunosorbent assay; Exact sciences and technology; galactose; glucuronic acid; hydrolysis; Larix laricina; molecular weight; monoclonal antibodies; Natural polymers; Physicochemistry of polymers; spectral analysis; Starch and polysaccharides; Structure; trees; wood; GlcA; Larix laricina; AG; Antibodies; AGP; MW; Arabinogalactan; SEC; Ara; MWCO; Gal; LAG; RI; Structure; ELISA; GLC; Molecular structure; Antigen antibody reaction; Softwood; Monoclonal antibody; Experimental study; Reactivity; Gymnospermae; Plant origin; Oside polymer; Coniferales; Spermatophyta; Molecular mass
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2011.07.006

► The structure of the arabinogalactan from Larix laricina has been elucidated. ► The 1,3,6-branched galactose core molecule is terminated by Galp, Arap, Araf or GlcAp. ► The molecular mass of the larch arabinogalactan has been determined to be 38,000Da. ► An ELISA method for quantification of larch arabinogalactan has been proposed. An arabinogalactan from wood of the larch tree (Larix laricina), which is a source of dietary fiber, is composed of d-galactose and l-arabinose in a 6:1 molar ratio accompanied by small amounts of d-glucuronic acid. The molecular mass has been determined to be 38kDa. Linkage analysis in combination with partial hydrolysis and spectroscopic investigations revealed a 1,3-linked Galp backbone, branched at C6 to 1,6-linked Galp side residues terminated by Galp, Arap, Araf or GlcpA. Monoclonal antibodies directed against a type-II-arabinogalactan from Echinacea purpurea showed cross reactivity with the larch arabinogalactan and have been used to develop an ELISA for quantification of this polysaccharide.