Host-guest recognition coupled with triple signal amplification endows an electrochemiluminescent biosensor with enhanced sensitivity

We demonstrate for the first time that host-guest recognition coupled with triple signal amplification endows an electrochemiluminescent (ECL) biosensor with enhanced sensitivity for uracil DNA glycosylase (UDG) assay. This biosensor exhibits good selectivity and extremely high sensitivity, and it c...

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Published inChemical communications (Cambridge, England) Vol. 56; no. 2; pp. 2971 - 2974
Main Authors Zhao, Min-hui, Cui, Lin, Zhang, Chun-yang
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 10.03.2020
Subjects
Amplification
Biosensing Techniques
Biosensors
Electrochemical Techniques
Electrochemiluminescence
Enzyme Inhibitors - chemistry
Enzyme Inhibitors - pharmacology
HeLa Cells
Humans
Luminescence
Recognition
Selectivity
Sensitivity enhancement
Uracil
Uracil-DNA Glycosidase - analysis
Uracil-DNA Glycosidase - antagonists & inhibitors
Uracil-DNA Glycosidase - metabolism
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Summary:We demonstrate for the first time that host-guest recognition coupled with triple signal amplification endows an electrochemiluminescent (ECL) biosensor with enhanced sensitivity for uracil DNA glycosylase (UDG) assay. This biosensor exhibits good selectivity and extremely high sensitivity, and it can be used to screen UDG inhibitors and measure the cellular UDG activity as well. Host-guest recognition coupled with triple signal amplification endows an electrochemiluminescent biosensor with enhanced sensitivity for uracil DNA glycosylase assay.
Bibliography:Electronic supplementary information (ESI) available: Details of experimental procedures and additional figures. See DOI
10.1039/c9cc09999a
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc09999a