Genome-wide Identification and Abiotic Stress Response Pattern Analysis of NF-Y Gene Family in Peanut (Arachis Hypogaea L.)

• Published in: Tropical plant biology Vol. 14; no. 4; pp. 329 - 344
• Main Authors: Wan, Qian, Luo, Lu, Zhang, Xiurong, Lv, Yuying, Zhu, Suqing, Kong, Lingrang, Wan, Yongshan, Liu, Fengzhen, Zhang, Kun
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2021; Springer Nature B.V
• Subjects: Abiotic stress; Abscisic acid; Amino acids; Arachis hypogaea; Biomedical and Life Sciences; Chromosomes; Developmental stages; Gene expression; Genes; Genomes; Legumes; Life Sciences; NF-Y gene; Osmotic stress; Pattern analysis; Peanuts; Phylogeny; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant Sciences; Plant species; Regulatory sequences; Salicylic acid; Transgenics; Y gene; Phylogenetic analysis; Expression patterns; NF-Y gene family; L.; Abiotic stress; Cultivated peanut
• ISSN: 1935-9756; 1935-9764
• DOI: 10.1007/s12042-021-09295-2

The nuclear factor Y (NF-Y) transcription factor (TF) family consists of three subfamilies NF-YA, NF-YB and NF-YC. Many studies have proven that NF-Y complex plays multiple essential roles in stress response in Arabidopsis and other plant species. However, little attention has been given to these genes in peanut. In this study, thirty-three AhNF-Y genes were identified in cultivated peanut and they were distributed on 16 chromosomes. A phylogenetic analysis of the NF-Y amino acid sequences indicated that the peanut NF-Y proteins were clustered in pairs at the end of the branches and showed high conservation with previous reported plant NF-Ys. Evolutionary history analysis showed that only segmental duplication contributed to expansion of this gene family. Analysis of the 1500-bp regulatory regions upstream the start codon showed that, except for AhNF-YB6 , peanut NF-Y s contained at least one abiotic stress response element in their regulatory region. Expression patterns of peanut NF-Ys in 22 tissues and developmental stages were analyzed. A few NF-Y s showed universal expression patterns, while most NF-Y s showed specific expression patterns. Through RNA-seq and qRT-PCR analyses, expression of six AhNF-Y genes was induced under salt stress in leaves or roots. In addition, AhNF-YA4 / 8 / 11 , NF-YB4 and NF-YC2 / 8 also responded to osmotic stress, ABA (abscisic acid) and salicylic acid (SA) treatment.