Rapid adaptive and acute stimulatory responses to low salinity stress in Pacific white shrimp (Litopenaeus vannamei): Insights from integrative transcriptomic and proteomic analysis

• Published in: Comparative biochemistry and physiology. Part D, Genomics & proteomics Vol. 48; p. 101149
• Main Authors: Lu, Huijie, Chen, Wenchun, Peng, Kai, Huang, Minwei, Zhao, Jichen, Chen, Xiaoying, Sun, Yuping, Ruan, Zhuohao, Li, Chaozheng, Liu, Ding, Liu, Zhenxing, Li, Huo, Zhang, Yong, Huang, Wen
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.12.2023
• Subjects: Acute low salinity stress; biochemistry; epithelium; euryhaline species; gene expression regulation; genome; genomics; hepatopancreas; histology; Litopenaeus vannamei; osmoregulation; Proteome; proteomics; Rapid adaption; salinity; salt stress; Transcriptome; transcriptomics; Rapid adaption; Acute low salinity stress; Proteome; Litopenaeus vannamei; Transcriptome
• ISSN: 1744-117X; 1878-0407; 1878-0407
• DOI: 10.1016/j.cbd.2023.101149

The Pacific white shrimp (Litopenaeus vannamei) is a euryhaline crustacean capable of tolerating a wide range of ambient salinity, but the strategies of hepatopancreas to rapid adaptive or acute stimulatory responses to extremely low salinity fluctuations remains unclear. In this study, we integrated transcriptomic and proteomic analyses on the hepatopancreas derived from rapid adaptative (RA) and acute stimulatory (AS) responses to extremely low salinity stress (0.3 ppt) to unveil specific regulatory mechanisms. The RA group displayed normal epithelial cells and tubule structures, while the AS group showed histological changes and lesions. A total of 754 and 649 differentially expressed genes (DEGs) were identified in RA and AS treatments, respectively. For proteome, a total of 206 and 66 differentially expressed proteins (DEPs) were obtained in the RA/CT and AS/CT comparison groups, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted among the DEGs and DEPs, revealing that metabolic related pathways were significantly enriched pathways in both comparison groups. In addition, correlation analysis of transcriptomic and proteomic results showed that 20 and 3 pairs of DEGs/DEPs were identified in RA vs. CT and AS vs. CT comparison groups, respectively. This study is the first report on the rapid adaptive and acute stimulatory transcriptomic and proteomic responses of L. vannamei to extremely low salinity, shedding light on the mechanisms underlying osmoregulation in euryhaline crustaceans. [Display omitted] •Metabolic related pathways were important in the response of L. vannamei to acute low salinity stress.•L. vannamei exhibited impressive rapid adaption to low salinity by adjusting lipid, carbohydrate, and amino acid metabolism.•Three hub genes, HMCL2, SCP β chain, and Obst-E, were identified.•Four candidate proteins, RELA, PMPCB, NDFUA6 and AMGO, were identified.