Development and validation of an analytical methodology based on solvent microextraction and UHPLC-MS/MS for determining bisphenols in honeys from different botanical origins

• Published in: Food chemistry Vol. 450; p. 139358
• Main Authors: Martín-Gómez, Beatriz, Valverde, Silvia, Bernal, José, Ares, Ana María
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 30.08.2024
• Subjects: Bisphenols; Food safety; Green analytical chemistry; Honey; Method validation; Sample treatment; UHPLC-MS/MS; BPBP; BPP; BF; MRM; SPE; BPFL; MS/MS; BPZ; SE; S/N; Bisphenols; BPPH; m/z; Green analytical chemistry; UHPLC; GC; HPLC; LOD; Sample treatment; BPAP; RSD; Method validation; BPs; AF; BPAF; LOQ; Honey; BPA; SML; UHPLC-MS/MS; BPC; BPB; BPE; SCI; BPF; Food safety; DLLME; BPM; BAGI
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2024.139358

A new analytical methodology was proposed to determine fourteen bisphenols in honeys from different botanical origins using ultra-high performance liquid chromatography-tandem mass spectrometry. A fast, efficient, environmentally-friendly and simple sample treatment (recoveries between 81% and 116%; matrix effect <20% for all studied compounds except for bisphenol E, F and S) was proposed, which involved a solvent microextraction with acetone and a small volume/amount of 1-hexanol. Chromatographic analysis (< 15 min) was performed in a Kinetex EVO C18 column under gradient elution mode. The method was validated in terms of selectivity, limits of detection (0.2–1.5 μg/kg) and quantification (0.5–4.7 μg/kg), linearity, matrix effect, trueness, and precision (relative standard deviation <17%). Finally, thirty honey samples were analyzed, revealing the presence of residues of nine bisphenols in some of them. However, quantification was possible only in two cases for bisphenol A, with a concentration of approximately 13 μg/kg. [Display omitted] •A new method was proposed and validated for determining 14 bisphenols in honeys.•Sample treatment consists of a solvent extraction with acetone and 1-hexanol.•Matrix effect was significant (> 20%) for three bisphenols (E, F and S).•The LOQs were lower than the SMLs established by current legislation.•Residues of nine bisphenols were detected in some of the analyzed honey samples.