Recombinant OB-Gene Product Reduces Food-Intake in Fasted Mice

• Published in: Biochemical and biophysical research communications Vol. 214; no. 1; pp. 131 - 136
• Main Authors: Rentsch, J., Levens, N., Chiesi, M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.09.1995
• Subjects: adipocytes; adipose tissue; amino acid sequences; Animals; Base Sequence; Cloning, Molecular; DNA Primers; Energy Intake - drug effects; Escherichia coli; Escherichia coli - genetics; Fasting; Feeding Behavior - drug effects; food intake; gene expression; genes; genetic transformation; Leptin; Male; Mice; Molecular Sequence Data; obesity; Obesity - genetics; Proteins - genetics; Proteins - pharmacology; Recombinant Proteins - genetics; Recombinant Proteins - pharmacology; Satiation - drug effects; satiety; satiety factors; white adipose tissue
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1995.2266

The ob-gene encodes for a protein of 167 amino acids which is expressed exclusively in white adipose tissue. The ob-gene product is probably released from adipocytes as a soluble hormone of 146 amino acids and has been proposed as a satiety factor. To test this hypothesis, the soluble portion of the ob-gene product devoid of signal sequence was expressed in E. coli and purified. The purified protein, which contains two Cys residues, was recovered from the periplasm in an oxidized form. After a single intravenous injection, the ob-gene product decreased food intake after fasting in normal mice. The results show that recombinant ob-gene product can be obtained in a functionally active conformation and provide direct proof that this protein is a satiety factor.