Hypoglycemic activity of phenols from Pleioblastus amarus (Keng) shells and its main chemical constituents identificatied using UHPLC-Q-TOF-MS

• Published in: Journal of functional foods Vol. 97; p. 105261
• Main Authors: Ma, Yuan, Meng, Ailian, Dai, Yemei, Yang, Huanhuan, Huang, Yongli, Liu, Xiaocui, Xian, Lirong, Yuan, Xue
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2022; Elsevier
• Subjects: Chemical composition analysis; Hypoglycemic activity; Phenols; Pleioblastus amarus (Keng) shell; Hypoglycemic activity; Phenols; Chemical composition analysis; Pleioblastus amarus (Keng) shell
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2022.105261

[Display omitted] •Pleioblastus amarus (Keng) shells was used as raw material to extract and separate polyphenols (PASP).•The effects of PASP on glucose consumption, intracellular glycogen content, hexokinase and pyruvate kinase activities in insulin-resistant HepG2 cells were measured.•Main chemical constituents of PASP was identified by UHPLC-Q-TOF, and 12 kinds of compounds were identified.•Four kinds of potential α-glucosidase inhibitors were screened by ultrafiltration affinity mass spectrometry. The aim of this study was to investigate the hypoglycemic activity of phenols extracted from Pleioblastus amarus (Keng) shells (PASs), and to isolate and identify the possible components.of which o-coumaric acid, ferulic acid, quercetin-3-0-rhamnoside, and apigenin-7-0-glucoside exhibited a strong affinity with α-glucosidase and were identified as potential The results revealed that, Pleioblastus amarus (Keng) shell phenols (PASP) displayed hypoglycemic activity in vitro, inhibiting α-glucosidase (IC50 = 0.158 ± 0.002 mg/mL) while improving glucose consumption in insulin-resistant (IR) HepG2 cells, as well as the intracellular glycogen content and hexokinase (HK) and pyruvate kinase (PK) activity. Twelve phenolic compounds were detected by UHPLC-Q-TOF-MS, α-glucosidase inhibitors (AGIs). This study suggests that PASs, as a by-product, has the potential to become a new raw material source for hypoglycemic functional food, providing an application prospect for commonly considered waste product.