Bioassay-guided purification of Lippia citriodora polyphenols with AMPK modulatory activity

•A total of 29 compounds were tentatively identified by HPLC-ESI-TOF-MS.•Semi-preparative HPLC provides purified compounds with AMPK modulation activity.•Fractions with pure verbascoside showed the highest activating capacity.•Lippia citriodora is a potential source for the obtainment of bioactive c...

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Published inJournal of functional foods Vol. 46; pp. 514 - 520
Main Authors Cádiz-Gurrea, María de la Luz, Olivares-Vicente, Mariló, Herranz-López, María, Arraez-Roman, David, Fernández-Arroyo, Salvador, Micol, Vicente, Segura-Carretero, Antonio
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.07.2018
Elsevier
Subjects
AMPK
HPLC
Lippia citriodora
Phenylpropanoids
Purification
Purification
Lippia citriodora
AMP
DAD
IBMX
Thr172
ADP
DMEM
BCS
BPC
DEX
FBS
RP-HPLC- ESI-TOF/MS
ROS
AMPK
Phenylpropanoids
AICAR
HPC
FA
HPLC
pAMPK
PVD
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Summary:•A total of 29 compounds were tentatively identified by HPLC-ESI-TOF-MS.•Semi-preparative HPLC provides purified compounds with AMPK modulation activity.•Fractions with pure verbascoside showed the highest activating capacity.•Lippia citriodora is a potential source for the obtainment of bioactive compounds for food industry. Recently, the relationship between nutrients and health is becoming since natural dietary products as polyphenols are being considered by their potential for the management of several diseases. We aimed to investigate the capacity of Lippia citriodora compounds to modulate AMP-activated protein kinase activity (AMPK) on a hypertrophic adipocyte model. HPLC semi-preparative purification method and reverse phase high performance liquid chromatography coupled to time-of-flight mass detection with electrospray ionization (RP-HPLC-ESI-TOF/MS) were used to obtain de compounds from L. citriodora extract. AMPK activity was measured on the hypertrophic 3T3-L1 adipocyte model by immunofluorescence microscopy. Four compounds of 29 total compounds have been tentatively identified in L. citriodora for the first time by HPLC-ESI-TOF-MS. Phenylpropanoids (verbascoside), iridoids (gardoside) and flavonoids (luteolin-7-diglucoronide) were the best candidates to account for activating AMPK capacity. The combination of specific polyphenols from L. citriodora, which showed strong activating AMPK capacity, could be an alternative in the management of obesity-associated diseases.
ISSN:1756-4646
2214-9414
DOI:10.1016/j.jff.2018.05.026