A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells

• Published in: Journal of functional foods Vol. 47; pp. 163 - 171
• Main Authors: Van den Abbeele, Pieter, Duysburgh, Cindy, Jiang, T. Alan, Rebaza, Marco, Pinheiro, Iris, Marzorati, Massimo
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.08.2018; Elsevier
• Subjects: Akkermansia; Obesity; Polyphenols; Prebiotic; SHIME; XOS; Polyphenols; Obesity; Akkermansia; XOS; Prebiotic; SHIME
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2018.05.053

[Display omitted] •The effect of a blend containing XOS and green coffee extract has been studied in the SHIME®.•Repeated daily doses of the blend led to an increased production of health-related metabolites.•Luminal amounts of bifidobacteria, lactobacilli and A. muciniphila were increased by the blend.•The blend showed immunomodulatory properties, especially in the distal colon. A validated in vitro gut model (i.e. SHIME®), coupled with co-cultures of epithelial cells and macrophages, was used to investigate the prebiotic potential and immune modulation of long-term administration of a xylooligosaccharides/polyphenols blend. The blend was well fermented along the entire colon leading to increased production of butyrate (+7 mmol/L) in the ascending colon; acetate, propionate and butyrate (approx. +4 mmol/L each) in the transverse colon; propionate (+4.1 mmol/L) in the descending colon. The treatment led to a decrease in ammonium and increased lactate levels in the ascending colon, which correlated with an increase in lactobacilli and bifidobacteria (+2.5 Log/mL). Additionally, the propionate-producing Akkermansia muciniphila increased in the lumen of the proximal colon (up to 7.15 log/mL). Finally, it was observed a protective effect on Caco-2 barrier function in the distal colon and pronounced immune activating properties. The generated data support a possible role in protecting against obesity-related adverse effects.