Morin ameliorates ovalbumin-induced allergic rhinitis via inhibition of STAT6/SOCS1 and GATA3/T-bet signaling pathway in BALB/c mice

• Published in: Journal of functional foods Vol. 55; pp. 391 - 401
• Main Authors: Liang, Kun, Kandhare, Amit D., Mukherjee-Kandhare, Anwesha A., Bodhankar, Subhash L., Xu, Dingyuan
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.04.2019; Elsevier
• Subjects: Allergic rhinitis; GATA3; Morin; Ovalbumin; SOCS1; STAT6; Ovalbumin; Morin; GATA; NLF; GATA3; T-bet; AR; IFN-γ; SOCS1; MLT; TNF-α; Allergic rhinitis; STAT6; IL’s; OVA; STAT-6
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2019.01.052

[Display omitted] •Allergic rhinitis (AR) was induced in Balb/c mice by using ovalbumin (OVA).•Morin (30 and 1000 mg/kg) significantly attenuated OVA-induced nasal symptoms.•Elevated levels of serum histamine, IgE, IgG1, and β-hexosaminidase decreased by morin.•Morin attenuated nasal lavage fluid IL-4, IL-5, IL-13, IL-17, and IFN-γ levels.•Morin attenuated GATA3, p-STAT6, SOCS1, and T-bet mRNA and protein expressions. Allergic rhinitis (AR) is an inflammatory immune disorder that occurs due to IgE-mediated hypersensitivity. Morin, a plant flavonoid exhibits inhibitory potential against IgE-mediated allergic diseases. To evaluate the efficacy and possible mechanism of action of morin against ovalbumin (OVA)-induced AR in BALB/c mice. AR was induced in mice by OVA (500 μl, i.p.) and challenged (5 μl per nostril) on the 21st day. Mice were treated with morin (10, 30 and 100 mg/kg, p.o.) for 21 days. OVA-challenge caused significant (p < 0.05) induction of nasal symptoms (rubbing, sneezing, and discharge) whereas administration of morin (30 and 100 mg/kg) significantly (p < 0.05) attenuated it as compared to AR control mice. The OVA-induced elevated levels of serum histamine, IgE, IgG1, β-hexosaminidase, nasal lavage fluid Th2 cytokines (IL-4, IL-5, IL-13, and IL-17), and decreased levels of nasal lavage fluid Th1 cytokines (IFN-γ) were significantly (p < 0.05) attenuated by morin. RT-PCR and western blot analysis revealed that morin treatment significantly (p < 0.05) down-regulated splenic mRNA and protein expressions of GATA3, p-STAT6, and SOCS1, significantly up-regulated (p < 0.05) T-bet expression as compared to AR control mice. OVA-induced alterations in nasal mucosa and spleen were significantly ameliorated (p < 0.05) by morin treatment. The findings of presented investigation demonstrated that morin exerts its anti-allergic effect against OVA-induced allergic rhinitis via inhibition of p-STAT6 that down-regulates expression of SOCS1 and GATA3/T-bet pathway which modulates the release of Th1/Th2 cytokines and IgE levels thus, inhibiting allergen-induced hypersensitivity.