Chemical Composition, Antioxidant and Antibacterial Activity of Different Extracts of Poplar Type Propolis

• Published in: Croatica Chemica Acta Vol. 91; no. 1; p. 81
• Main Authors: Masek, Tomislav, Perin, Natasa, Racane, Livio, Cindric, Maja, Paljetak, Hana Cipcic, Peric, Mihaela, Matijasic, Mario, Verbanac, Donatella, Radic, Bozo, Suran, Jelena, Starcevic, Kristina
• Format: Journal Article Paper
• Language: English
• Published: Croatica Chemica Acta 01.01.2018; Hrvatsko kemijsko društvo
• Subjects: Amino acids; Antibacterial agents; Antioxidant activity; Antioxidants (Nutrients); Esters; Isoflavones; Propolis
• ISSN: 0011-1643; 1334-417X
• DOI: 10.5562/cca3298

Chemical composition, antioxidant activity and in vitro antibacterial activity of propolis from the central, continental part of Croatia were studied. Propolis hydro-ethanolic extracts (PHEE), prepared using three different methods and two solvent mixtures contained high amounts of flavonoids (20.95-28.11% TIC), aromatic acids (8.17-15.91% TIC) and their esters (9.27-11.91% TIC). The PHEE obtained in this study showed high antioxidant activity (DPPH [IC.sub.50] values from 9.96-19.95 [micro]g/ml and FRAP 38.0-41.9 mM [Fe.sup.2+]/mg PHEE). Despite differences in composition, the PHEE samples exhibited significant antibacterial activities, affecting tested strains of Staphylococcus aures, Enterococcus faecalis, Escherichia coli and Moraxella catarrhalis. The use of different solvent ratio and extraction procedures selectively increases or decreases the content of specific propolis components in the extract which can have a beneficial effect on the application of propolis extracts. Keywords: Propolis, Antioxidant activity, Antibacterial agents.