Regulation of Apterous activity in Drosophila wing development

• Published in: Development (Cambridge) Vol. 128; no. 22; pp. 4615 - 4622
• Main Authors: Weihe, Ulrich, Milán, Marco, Cohen, Stephen M.
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Limited 15.11.2001
• Subjects: Animals; Apterous protein; Binding, Competitive; Chip protein; Cysteine Endopeptidases - metabolism; Drosophila; Drosophila Proteins; Gene Expression Regulation, Developmental; Homeodomain Proteins - chemistry; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Larva; LIM-Homeodomain Proteins; Models, Genetic; Multienzyme Complexes - metabolism; Nuclear Proteins - chemistry; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Proteasome Endopeptidase Complex; Protein Binding; Protein Structure, Tertiary; Recombinant Fusion Proteins - metabolism; Transcription Factors - chemistry; Transcription Factors - genetics; Transcription Factors - metabolism; Wings, Animal - growth & development
• ISSN: 0950-1991; 1477-9129
• DOI: 10.1242/dev.128.22.4615

Apterous is a LIM-homeodomain protein that confers dorsal compartment identity in Drosophila wing development. Apterous activity requires formation of a complex with a co-factor, Chip/dLDB. Apterous activity is regulated during wing development by dLMO, which competes with Apterous for complex formation. Here, we present evidence that complex formation between Apterous, Chip and DNA stabilizes Apterous protein in vivo. We also report that a difference in the ability of Chip to bind the LIM domains of Apterous and dLMO contributes to regulation of activity levels in vivo.