PEROXIDASE-LINKED ASSAY FOR DETECTION OF ANTIBODIES AGAINST BOVINE LEUKOSIS VIRUS

• Published in: Journal of immunoassay & immunochemistry Vol. 34; no. 4; pp. 376 - 383
• Main Authors: de Castro, Clarissa C., Nunes, Cristina F., Finger, Paula F., Siedler, Bianca S., Dummer, Luana, de Lima, Marcelo, Leite, Fábio P. L., Fischer, Geferson, Vargas, Gilberto D'A., Hübner, Silvia de O.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 02.10.2013
• Subjects: Animals; antibodies; Antibodies, Viral - blood; bovine leukosis virus; Cattle; diagnostic; Enzootic Bovine Leukosis - diagnosis; Enzootic Bovine Leukosis - immunology; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Immunodiffusion - methods; immunoperoxidase; Leukemia Virus, Bovine - immunology; Peroxidase - immunology; Sensitivity and Specificity; serology; test
• ISSN: 1532-1819; 1532-4230
• DOI: 10.1080/15321819.2012.747442

A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27 samples found to be positive by PLA. PLA showed 100% of sensitivity when compared with AGID and ELISA. Specificity was 64.8% and 78%, respectively (kappa coefficients were 0.70 and 0.83). These findings indicate that PLA can be used as an alternative method for the diagnosis of BLV infection in cattle.