Small-angle X-ray scattering studies of rabbit reticulocyte ribosomes using a Berreman-type point-focusing monochromator

Small-angle X-ray scattering patterns were obtained from rabbit reticulocyte ribosomes using a Berreman-type point-focusing single quartz crystal monochromator. These patterns are interpreted to determine the radius of gyration of 105 Å to about 2% and an estimate of the molecular weight. No evidenc...

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Bibliographic Details
Published inJournal of ultrastructure research Vol. 11; no. 3; pp. 363 - 373
Main Author Dibble, William E.
Format Journal Article
LanguageEnglish
Published United States Elsevier B.V 01.10.1964
Subjects
Animals
Crystallography
Lagomorpha
Old Medline
Rabbits
Radiometry
Reticulocytes
Ribosomes
X-Rays
RADIOMETRY
RIBOSOMES
RABBITS
CRYSTALLOGRAPHY
RETICULOCYTES
EXPERIMENTAL LAB STUDY
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Summary:Small-angle X-ray scattering patterns were obtained from rabbit reticulocyte ribosomes using a Berreman-type point-focusing single quartz crystal monochromator. These patterns are interpreted to determine the radius of gyration of 105 Å to about 2% and an estimate of the molecular weight. No evidence of secondary scattering maxima was observed. An investigation of approximate volume changes between two ionic strengths was made with negative results. The electron density of the sample is approximated by a Gaussian consistent with the data with a cutoff at 170 Å, giving in connection with electron microscope data of C. Hall a hydration of 2.7 g water per gram ribosome.
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content type line 23
ISSN:0022-5320
1878-2345
DOI:10.1016/S0022-5320(64)90039-5