Use of enzyme-labelled protein G assay for the detection of anti Borrelia burgdorferi antibodies in wild animal sera

• Published in: European journal of epidemiology Vol. 12; no. 5; p. 515
• Main Authors: Deruaz, D, Eid, P, Deruaz, J, Sempéré, A, Bourgouin, C, Rodhain, F, Pérez-Eid, C
• Format: Journal Article
• Language: English
• Published: Netherlands 01.10.1996
• Subjects: Animals; Animals, Wild - immunology; Antibodies, Bacterial - blood; Borrelia burgdorferi Group - immunology; Deer - immunology; Enzyme-Linked Immunosorbent Assay; France; Hemagglutination Tests; Immunoglobulin G - immunology; Lyme Disease - immunology; Lyme Disease - veterinary; Nucleic Acid Amplification Techniques; Reproducibility of Results; France
• ISSN: 0393-2990
• DOI: 10.1007/BF00144006

A modified ELISA was developed for the detection of anti-Borrelia burgdorferi (Bb) IgG antibodies in wild animal sera based on an Enzyme-Labelled-protein G Assay (ELGA). Microplates were coated with an extract of Bb sensu stricto strain (SVI) as antigen. Specific antibodies of the serum samples were detected by a peroxidase-labelled-protein G. Using comparative immunodiagnosis by means of a passive hemagglutination test (HA), ELGA was tested on 82 roe-deer blood samples. A correlation was found between the two methods (r = 0.66). Good reproducibility of titers was observed by ELGA technique. A minimal cross-reactivity was discovered with Leptospira. ELGA could facilitate the recognition of specific antibodies in collections of wild animal sera.