Association of Long-Chain Noncoding RNA H19 and MEG3 Gene Polymorphisms and Their Interaction with Risk of Osteoarthritis in a Chinese Han Population

• Published in: Genetic testing and molecular biomarkers Vol. 24; no. 6; p. 328
• Main Authors: Wang, Huang, Li, Jian, Cheng, Ye, Yao, Jun
• Format: Journal Article
• Language: English
• Published: United States 01.06.2020
• Subjects: Adult; Aged; Aged, 80 and over; Alleles; Asian Continental Ancestry Group - genetics; Case-Control Studies; Ethnic Groups - genetics; Female; Gene Expression Regulation - genetics; Gene Frequency - genetics; Genetic Predisposition to Disease; Genotype; Humans; Male; MicroRNAs - genetics; Middle Aged; Osteoarthritis - genetics; Polymorphism, Single Nucleotide - genetics; Risk Factors; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; MEG3; H19; long-chain noncoding RNA; single nucleotide polymorphism; osteoarthritis
• ISSN: 1945-0257
• DOI: 10.1089/gtmb.2019.0230

To analyze the association of single nucleotide polymorphisms (SNPs) in the long-chain noncoding RNAs (lncRNAs) and maternal expressed gene 3 ( with the risk of osteoarthritis (OA) in a Chinese Han population. A total of 230 patients with OA and 230 control subjects were recruited. Sanger sequencing was used to analyze the genotypes of the gene rs217727 and rs3741219 loci and the gene rs7158663 locus. Quantitative real-time reverse transcription-polymerase chain reaction was used to determine the levels of the microRNAs (miRNA) hsa-miR-4804-5p, hsa-miR-8071, hsa-miR-8072, hsa-miR-3960, hsa-miR-4307, and hsa-miR-1265 in the plasma. The risk of OA was significantly increased by the A allele of the rs217727 locus of (odds ratio = 1.27, 95% confidence interval [CI]: 1.09-1.42,  = 0.001). The gene rs3741219 locus SNP had no significant correlation with the risk of OA (  > 0.05). The risk of OA in subjects carrying the rs7158663 locus A allele of was 1.32 times higher compared with those carrying the G allele (95% CI: 1.16-1.50,  < 0.01). SNP rs217727 of was associated with the plasma levels of lncRNA , hsa-miR-4804-5p, hsa-miR-8071, hsa-miR-8072, and hsa-miR-3960, and SNP rs7158663 in was associated with the plasma levels of lncRNA , hsa-miR-4307, and hsa-miR-1265. Specific SNPs of the rs217727 and rs7158663 loci are associated with the risk of OA. The possible underlying molecular mechanisms, which remain to be confirmed, are their likely influence on the expression levels of the lncRNA and , which in turn alters the expression level of their target miRNAs.