Genetic differences among ‘Luotian-tianshi’ (Diospyros kaki Thunb.) genotypes native to China revealed by ISSR and IRAP markers

• Published in: Scientia horticulturae Vol. 137; pp. 75 - 80
• Main Authors: Yuan, Luxia, Zhang, Qinglin, Guo, Dayong, Luo, Zhengrong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2012; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; cluster analysis; cultivars; Diospyros kaki; Diospyros kaki Thunb. ‘Luotian-tianshi’; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic differences; genetic markers; Genetic resources, diversity; genetic variation; Genetics and breeding of economic plants; genotype; IRAP; ISSR; mountains; mutation; persimmons; phylogeny; Plant material; proliferating cell nuclear antigen; Asia; China; Genetic differences; IRAP; ISSR; Diospyros kaki Thunb. ‘Luotian-tianshi’; Diospyros kaki; Genetic variability; Horticulture; Genotype; Inter-retrotransposon amplified polymorphism marker; Diospyros kaki Thunb.'Luotian-tianshi'; Ebenaceae; Dicotyledones; Microsatellite DNA; Angiospermae; Spermatophyta; Fruit tree
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2012.01.027

► We analyzed the genetic differences among 43 ‘Luotian-tianshi’ genotypes native to China using ISSR and IRAP markers. ► We predicted that ‘Luotian-tianshi’ would be a cultivar group rather than a single cultivar. ► ISSR marker would be more suitable for analyzing the distinct genetic differences to select special individuals, and IRAP for studying population genetic diversity or the origin and spreading process of ‘Luotian-tianshi’. ‘Luotian-tianshi’ (Diospyros kaki Thunb.), which has been geographically distributed only in Dabieshan Mountain around the junction of Hubei, Henan and Anhui Province in central China, is believed to be the first PCNA (pollination-constant non-astringent) persimmon native to China. An informative scenario about the genetic diversity and phylogenetic relationships of this native PCNA cultivar is still lacking. To understand the genetic diversity, we have effectively utilized ISSR and IRAP markers to predict the genetic differences among 43 ‘Luotian-tianshi’ genotypes collected from three major producing areas in China. A total number of 280 amplicons were observed ranging between 250 and 2000bp using ISSR and IRAP markers. ISSR markers (100%) revealed a higher polymorphism than the IRAP markers (97.33%). On the basis of cluster analysis in the UPGMA dendrogram, we observed that in case of ISSR the genotypes were clustered into two clusters, whereas three main clusters were obtained by IRAP, indicating that ‘Luotian-tianshi’ genotypes existed the genetic differences and some could be potential germplasms because of the significant genetic differences, like Acc11, Acc36, Acc40 and Acc41. ‘Luotian-tianshi’ was a cultivar group rather than a single cultivar, and mutation and crossover might have happened in the evolution.