Atomic force microscopy of pea starch: Granule architecture of the rug3-a, rug4-b, rug5-a and lam-c mutants
Atomic force microscopy has been used to visualise the internal structures of sectioned, encased starch granules isolated from near-isogenic pea starch mutants ( rug3-a, rug4-b, rug5-a, and lam-c). A mutation at the locus rug4 was found to have little effect on the granule ultrastructure. However, m...
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Published in | Carbohydrate polymers Vol. 65; no. 1; pp. 64 - 74 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Ltd
10.07.2006
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | Atomic force microscopy has been used to visualise the internal structures of sectioned, encased starch granules isolated from near-isogenic pea starch mutants (
rug3-a,
rug4-b,
rug5-a, and
lam-c). A mutation at the locus
rug4 was found to have little effect on the granule ultrastructure. However, mutations at
rug3 and
lam, which give rise to low-amylose starches, led to granules that showed banding (growth rings) in which individual blocklets could not easily be seen. High-amylose (
rug5) mutants formed granules ranging in shape from simple ellipsoids through to quite complex, convoluted structures. The internal granule structure was found to be heterogeneous. In some regions, normal banding was visible and the underlying ‘hard’ blocklets were embedded in a ‘soft’ matrix. In other regions of the granule, the banding structure was absent and the matrix in which the blocklets are embedded contained a fine hard network structure. It is proposed that this fine, hard structure is due to the presence of a crystalline amylose network. |
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Bibliography: | http://dx.doi.org/10.1016/j.carbpol.2005.12.016 |
ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2005.12.016 |