Identification of hypoxia-responsive genes in a dopaminergic cell line by subtractive cDNA libraries and microarray analysis

Transplantation of dopamine-secreting cells harvested from fetal mesencephalon directly into the striatum has had limited success as a therapy for Parkinson's disease. A major problem is that the majority of the cells die during the first 3 weeks following transplantation. Hypoxia in the tissue...

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Published inParkinsonism & related disorders Vol. 7; no. 3; pp. 273 - 281
Main Authors Beitner-Johnson, D, Seta, K, Yuan, Y, Kim, H.-W, Rust, R.T, Conrad, P.W, Kobayashi, S, Millhorn, D.E
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.07.2001
Subjects
Carotid body
Genomics
Oxygen/pheochromocytoma
Type I cells
Tyrosine hydroxylase
Oxygen/pheochromocytoma
Tyrosine hydroxylase
Type I cells
Carotid body
Genomics
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Summary:Transplantation of dopamine-secreting cells harvested from fetal mesencephalon directly into the striatum has had limited success as a therapy for Parkinson's disease. A major problem is that the majority of the cells die during the first 3 weeks following transplantation. Hypoxia in the tissue surrounding the graft is a potential cause of the cell death. We have used subtractive cDNA libraries and microarray analysis to identify the gene expression profile that regulates tolerance to hypoxia. An improved understanding of the molecular basis of hypoxia-tolerance may allow investigators to engineer cells that can survive in the hypoxic environment of the brain parenchyma following transplantation.
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ISSN:1353-8020
1873-5126
DOI:10.1016/S1353-8020(00)00070-5